Cholinergic Neuronotrophic Factors: Intraocular Distribution of Trophic Activity for Ciliary Neurons

R. Adler; K. B. Landa; M. Manthorpe; S. Varon

doi:10.1126/science.451576

journal article Jun 29, 1979

Cholinergic Neuronotrophic Factors: Intraocular Distribution of Trophic Activity for Ciliary Neurons

R. Adler K. B. Landa M. Manthorpe S. Varon

Science Vol. 204 No. 4400 pp. 1434-1436 · American Association for the Advancement of Science (AAAS)

View at Publisher Save 10.1126/science.451576

Abstract

Chick ciliary ganglionic neurons require an interaction with their peripheral targets for survival during a critical period of their embryonic development in vivo. It has recently been shown that survival of these neurons in dissociated cell cultures is supported by extract from whole chick embryo. In this study, an assay system based on microwell cultures of ciliary ganglionic neurons was used to demonstrate that a very rich source of trophic factor for them is the intraocular target tissues they innervate. Out of 8000 trophic units present in a 12-day embryo, 2500 were contained in the eye. A subdissection of the eye showed its activity to be localized in a fraction containing the ciliary body and choroid coat, with a specific activity almost 20-fold higher than that of the whole embryo. This selective intraocular distribution at a time when survival or death of ciliary ganglionic neurons is decided in vivo suggests that this soluble factor may be involved in the normal development of the ciliary ganglion.

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References

Details

Published: Jun 29, 1979
Vol/Issue: 204(4400)
Pages: 1434-1436

Authors

R

R. Adler

Departments of Biology and Neuroscience, University of California at San Diego, La Jolla 92093

K

K. B. Landa

Departments of Biology and Neuroscience, University of California at San Diego, La Jolla 92093

M

M. Manthorpe

Departments of Biology and Neuroscience, University of California at San Diego, La Jolla 92093

S

S. Varon

Departments of Biology and Neuroscience, University of California at San Diego, La Jolla 92093

Cite This Article

R. Adler, K. B. Landa, M. Manthorpe, et al. (1979). Cholinergic Neuronotrophic Factors: Intraocular Distribution of Trophic Activity for Ciliary Neurons. Science, 204(4400), 1434-1436. https://doi.org/10.1126/science.451576

Cholinergic Neuronotrophic Factors: Intraocular Distribution of Trophic Activity for Ciliary Neurons

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