Protein Sequence Analysis: Automated Microsequencing

Michael W. Hunkapiller; Leroy E. Hood

doi:10.1126/science.6687410

journal article Feb 11, 1983

Protein Sequence Analysis: Automated Microsequencing

Michael W. Hunkapiller Leroy E. Hood

Science Vol. 219 No. 4585 pp. 650-659 · American Association for the Advancement of Science (AAAS)

View at Publisher Save 10.1126/science.6687410

Abstract

The automated microsequencing of proteins can now be carried out at the 5- to 10-picomoles (submicrogram) level on polypeptides obtained directly from one- and two-dimensional gel electrophoresis. The techniques are applicable to polypeptides ranging in size from small peptides (less than 10 residues) to large proteins (more than 1000 residues).

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References

38

[1]

NATURE 275: 90 (1978). 10.1038/275090a0

[2]

BALLOU, B, HLA MEMBRANE ANTIGENS - SEQUENCING BY INTRINSIC RADIOACTIVITY, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 73: 4487 (1976).

[3]

BEAUCAGE, S.L., DEOXYNUCLEOSIDE PHOSPHORAMIDITES - A NEW CLASS OF KEY INTERMEDIATES FOR DEOXYPOLYNUCLEOTIDE SYNTHESIS, TETRAHEDRON LETTERS 22: 1859 (1981). 10.1016/s0040-4039(01)90461-7

[4]

Protection against foot-and-mouth disease by immunization with a chemically synthesized peptide predicted from the viral nucleotide sequence

James L. Bittle, Richard A. Houghten, Hannah Alexander et al.

Nature 1982 10.1038/298030a0

[5]

BRIDGEN, J, SOLID-PHASE SEQUENCING - NEW SUPPORT FOR HIGH SENSITIVITY DEGRADATION OF PEPTIDES AND PROTEINS, FEBS LETTERS 50: 159 (1975). 10.1016/0014-5793(75)80479-0

[6]

BURSTEIN, Y, AMINO-ACID SEQUENCE OF NH2-TERMINAL EXTRA PIECE SEGMENTS OF PRECURSORS OF MOUSE IMMUNOGLOBULIN LAMBDA-1-TYPE AND KAPPA-TYPE LIGHT-CHAINS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 74: 716 (1977).

[7]

CHANG, J.Y., MICRO-SEQUENCE ANALYSIS OF PEPTIDES AND PROTEINS USING 4-NN-DIMETHYLAMINOAZOBENZENE 4'-ISOTHIOCYANATE-PHENYLISOTHIOCYANATE DOUBLE COUPLING METHOD, FEBS LETTERS 93: 205 (1978). 10.1016/0014-5793(78)81105-3

[8]

COLIGAN, J.E., PRIMARY STRUCTURE OF A MURINE TRANSPLANTATION ANTIGEN, NATURE 291: 35 (1981). 10.1038/291035a0

[9]

EDMAN, P, ON THE MECHANISM OF THE PHENYL ISOTHIOCYANATE DEGRADATION OF PEPTIDES, ACTA CHEMICA SCANDINAVICA 10: 761 (1956). 10.3891/acta.chem.scand.10-0761

[10]

A Protein Sequenator

P. Edman, G. Begg

European Journal of Biochemistry 1967 10.1111/j.1432-1033.1967.tb00047.x

[11]

Fischli, W., Proceedings of the National Academy of Sciences of the United States of America 79: 5435 (1982).

[12]

HEWICK, R.M., A GAS-LIQUID SOLID-PHASE PEPTIDE AND PROTEIN SEQUENATOR, JOURNAL OF BIOLOGICAL CHEMISTRY 256: 7990 (1981). 10.1016/s0021-9258(18)43377-7

[13]

HUNKAPILLER, M.W., DIRECT MICROSEQUENCE ANALYSIS OF POLYPEPTIDES USING AN IMPROVED SEQUENATOR, A NON-PROTEIN CARRIER (POLYBRENE), AND HIGH-PRESSURE LIQUID-CHROMATOGRAPHY, BIOCHEMISTRY 17: 2124 (1978). 10.1021/bi00604a016

[14]

Hunkapiller, M. W., Methods in Enzymology 91: 227 (1983). 10.1016/s0076-6879(83)91019-4

[15]

10.1126/science.7352258

[16]

JACOBS, J.W., HIGH SENSITIVITY AUTOMATED SEQUENCE DETERMINATION OF POLYPEPTIDES, JOURNAL OF BIOLOGICAL CHEMISTRY 250: 3629 (1975). 10.1016/s0021-9258(19)41443-9

[17]

LAURSEN, R.A., SOLID-PHASE EDMAN DEGRADATION - AUTOMATIC PEPTIDE SEQUENCER, EUROPEAN JOURNAL OF BIOCHEMISTRY 20: 89 (1971). 10.1111/j.1432-1033.1971.tb01366.x

[18]

MAHONEY, W.C., SEPARATION OF LARGE DENATURED PEPTIDES BY REVERSE PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY - TRIFLUOROACETIC-ACID AS A PEPTIDE SOLVENT, JOURNAL OF BIOLOGICAL CHEMISTRY 255: 11199 (1980). 10.1016/s0021-9258(19)70275-0

[19]

MATTEUCCI, M.D., NUCLEOTIDE CHEMISTRY .4. SYNTHESIS OF DEOXYOLIGONUCLEOTIDES ON A POLYMER SUPPORT, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 103: 3185 (1981). 10.1021/ja00401a041

[20]

MENDEZ, E, REGENERATION OF AMINO-ACIDS FROM THIAZOLINONES FORMED IN EDMAN DEGRADATION, ANALYTICAL BIOCHEMISTRY 68: 47 (1975). 10.1016/0003-2697(75)90677-6

[21]

NOYES, B.E., DETECTION AND PARTIAL SEQUENCE-ANALYSIS OF GASTRIN MESSENGER-RNA BY USING AN OLIGODEOXYNUCLEOTIDE PROBE, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 76: 1770 (1979).

[22]

OROSZLAN, S, SOLID PHASE METHODS: 179 (1975).

[23]

PISANO, J.J., ADVANCES IN GAS-CHROMATOGRAPHIC ANALYSIS OF AMINO-ACID PHENYLTHIOHYDANTOINS AND METHYLTHIOHYDANTOINS, ANALYTICAL BIOCHEMISTRY 45: 43 (1972). 10.1016/0003-2697(72)90006-1

[24]

10.1126/science.7384786

[25]

SILVER, J, CONTEMPORARY TOPICS 5: 35 (1976).

[26]

SILVER, J, STRUCTURE AND EVOLUTION OF TRANSPLANTATION ANTIGENS - PARTIAL AMINO-ACID-SEQUENCES OF H-2K AND H-2D ALLOANTIGENS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 73: 599 (1976).

[27]

SMITHIES, O, QUANTITATIVE PROCEDURES FOR USE WITH EDMAN-BEGG SEQUENATOR - PARTIAL SEQUENCES OF 2 UNUSUAL IMMUNOGLOBULIN LIGHT CHAINS, RZF AND SAC, BIOCHEMISTRY 10: 4912 (1971). 10.1021/bi00802a013

[28]

STEINMETZ, M, A MOLECULAR MAP OF THE IMMUNE-RESPONSE REGION FROM THE MAJOR HISTOCOMPATIBILITY COMPLEX OF THE MOUSE, NATURE 300: 35 (1982). 10.1038/300035a0

[29]

10.1038/287801a0

[30]

TARR, G.E., METHODS PROTEIN SEQU: 223 (1982).

[31]

TARR, G.E., POLY-QUARTERNARY AMINES PREVENT PEPTIDE LOSS FROM SEQUENATORS, ANALYTICAL BIOCHEMISTRY 84: 622 (1978). 10.1016/0003-2697(78)90086-6

[32]

WALKER, J.E., SOLID-PHASE SEQUENCE-ANALYSIS OF POLYPEPTIDES ELUTED FROM POLYACRYLAMIDE GELS - AN AID TO INTERPRETATION OF DNA-SEQUENCES EXEMPLIFIED BY THE ESCHERICHIA-COLI-UNC OPERON AND BACTERIOPHAGE-LAMBDA, EUROPEAN JOURNAL OF BIOCHEMISTRY 123: 253 (1982). 10.1111/j.1432-1033.1982.tb19761.x

[33]

Walter, G., Proceedings of the National Academy of Sciences of the United States of America 77: 5197 (1980).

[34]

WILSON, K.J., METHODS PROTEIN SEQU: 401 (1982).

[35]

WITTMANNLIEBOLD, B, DEVICE COUPLED TO A MODIFIED SEQUENATOR FOR AUTOMATED CONVERSION OF ANILINOTHIAZOLINONES INTO PTH AMINO-ACIDS, ANALYTICAL BIOCHEMISTRY 75: 621 (1976). 10.1016/0003-2697(76)90117-2

[36]

WITTMANNLIEBOLD, B, AMINO-ACID SEQUENCE STUDIES ON 10 RIBOSOMAL-PROTEINS OF ESCHERICHIA-COLI WITH AN IMPROVED SEQUENATOR EQUIPPED WITH AN AUTOMATIC CONVERSION DEVICE, HOPPE-SEYLERS ZEITSCHRIFT FUR PHYSIOLOGISCHE CHEMIE 354: 1415 (1973). 10.1515/bchm2.1973.354.2.1415

[37]

WITTMANLIEBOLD, B, STUDIES ON PRIMARY STRUCTURE OF 14 PROTEINS FROM LARGE SUBUNIT OF ESCHERICHIA-COLI RIBOSOMES WITH AN IMPROVED PROTEIN SEQUENATOR AND WITH MASS-SPECTROMETRY, JOURNAL OF SUPRAMOLECULAR STRUCTURE 3: 426 (1975). 10.1002/jss.400030505

[38]

ZIMMERMAN, C.L., RAPID ANALYSIS OF AMINO-ACID PHENYLTHIOHYDANTOINS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, ANALYTICAL BIOCHEMISTRY 77: 569 (1977). 10.1016/0003-2697(77)90276-7

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References

Details

Published: Feb 11, 1983
Vol/Issue: 219(4585)
Pages: 650-659

Authors

M

Michael W. Hunkapiller

Division of Biology, California Institute of Technology, Pasadena 91125.

L

Leroy E. Hood

Division of Biology, California Institute of Technology, Pasadena 91125.

Cite This Article

Michael W. Hunkapiller, Leroy E. Hood (1983). Protein Sequence Analysis: Automated Microsequencing. Science, 219(4585), 650-659. https://doi.org/10.1126/science.6687410

Protein Sequence Analysis: Automated Microsequencing

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