journal article
Aug 15, 2006
The Nitric Oxide-Responsive Regulator NsrR Controls ResDE-Dependent Gene Expression
Abstract
ABSTRACTThe ResD-ResE signal transduction system is essential for aerobic and anaerobic respiration inBacillus subtilis. ResDE-dependent gene expression is induced by oxygen limitation, but full induction under anaerobic conditions requires nitrite or nitric oxide (NO). Here we report that NsrR (formerly YhdE) is responsible for the NO-dependent up-regulation of the ResDE regulon. The null mutation ofnsrRled to aerobic derepression ofhmp(flavohemoglobin gene) partly in a ResDE-independent manner. In addition to its negative role in aerobichmpexpression, NsrR plays an important role under anaerobic conditions for regulation of ResDE-controlled genes, includinghmp. ResDE-dependent gene expression was increased by thensrRmutation in the absence of NO, but the expression was decreased by the mutation when NO was present. Consequently,B. subtiliscells lacking NsrR no longer sense and respond to NO (and nitrite) to up-regulate the ResDE regulon. Exposure to NO did not significantly change the cellular concentration of NsrR, suggesting that NO likely modulates the activity of NsrR. NsrR is similar to the recently described nitrite- or NO-sensitive transcription repressors present in various bacteria. NsrR likely has an Fe-S cluster, and interaction of NO with the Fe-S center is proposed to modulate NsrR activity.
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References
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References
Details
- Published
- Aug 15, 2006
- Vol/Issue
- 188(16)
- Pages
- 5878-5887
- License
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Authors
Cite This Article
Michiko M. Nakano, Hao Geng, Shunji Nakano, et al. (2006). The Nitric Oxide-Responsive Regulator NsrR Controls ResDE-Dependent Gene Expression. Journal of Bacteriology, 188(16), 5878-5887. https://doi.org/10.1128/jb.00486-06
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