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journal article Jun 15, 2013

Exercise and the platelet activator calcium chloride both influence the growth factor content of platelet-rich plasma (PRP): overlooked biochemical factors that could influence PRP treatment

Bruce Hamilton Johannes L Tol Wade Knez Hakim Chalabi
British Journal of Sports Medicine Vol. 49 No. 14 pp. 957-960 · BMJ
View at Publisher Save 10.1136/bjsports-2012-091916
Abstract
BackgroundThere is strong evidence that exercise affects platelet haemostasis factors, but this potential effect on growth factor concentrations in platelet-rich plasma (PRP) has never been studied. In addition, there is a paucity of studies focusing on the effects of activating agents used in conjunction with PRP. The first aim of this study was to evaluate the effect of exercise on platelet and platelet-derived growth factors (PDGF)-AB, hepatocyte growth factor (HGF), insulin-like growth factor-1 (IGF-1) and vascular endothelial growth factor (VEGF) concentrations in PRP. The second aim was to study the effect of the activating agent calcium chloride (CaCl2) on growth factor concentration in relation to different exercise states.MethodsControlled laboratory study. Ten healthy participants performed 1 h of submaximal exercise with blood being withdrawn immediately pre, post and 18 h following. PRP was prepared in each condition in both an activated CaCl2and non-activated form. Concentrations of PDGF-AB, HGF, IGF-1 and VEGF were evaluated using standard ELISA systems.ResultsExercise had no significant effect on platelet concentration, but significantly suppressed both VEGF and PDGF-AB concentrations. Exercise state had no significant effect on IGF-1 or HGF concentration. Activation with CaCl2resulted in a significant increase in PDGF-AB and IGF-1 concentrations, unchanged VEGF and significantly reduced HGF concentrations.ConclusionsExercise significantly impacts on PDGFs in PRP with significantly reduced concentrations of VEGF and PDFG-AB. Furthermore, the activation of PRP with CaCl2results in a differentiated GF release from platelets. These relevant factors can potentially influence outcome in daily clinical practice and are recommended to be accounted for in future study design.
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Metrics
56
Citations
37
References
Details
Published
Jun 15, 2013
Vol/Issue
49(14)
Pages
957-960
Authors
B
Bruce Hamilton
J
Johannes L Tol
W
Wade Knez
H
Hakim Chalabi
Cite This Article
Bruce Hamilton, Johannes L Tol, Wade Knez, et al. (2013). Exercise and the platelet activator calcium chloride both influence the growth factor content of platelet-rich plasma (PRP): overlooked biochemical factors that could influence PRP treatment. British Journal of Sports Medicine, 49(14), 957-960. https://doi.org/10.1136/bjsports-2012-091916
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