Modified Dimethyl Sulfoxide (DMSO) Extraction for Chlorophyll Analysis of Phytoplankton

B. K. Burnison

doi:10.1139/f80-095

journal article Apr 01, 1980

Modified Dimethyl Sulfoxide (DMSO) Extraction for Chlorophyll Analysis of Phytoplankton

B. K. Burnison

Canadian Journal of Fisheries and Aquatic Sciences Vol. 37 No. 4 pp. 729-733 · Canadian Science Publishing

View at Publisher Save 10.1139/f80-095

Abstract

This new extraction procedure eliminates the need for grinding glass fiber filters by heating the filter in DMSO (4 mL) for 10 min at 65 °C and subsequently filtering or centrifuging the sample after dilution with 90% acetone (6 mL). Scenedesmus and Selenastrum species gave about 20% higher concentrations of chlorophyll a using this method as compared to the standard 90% acetone procedure or grinding in DMSO only. The difference between heated DMSO and 90% acetone was reduced when natural phytoplankton were analyzed.Key words: chlorophyll, Chlorophyta, dimethyl sulfoxide, acetone, extraction

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Published: Apr 01, 1980
Vol/Issue: 37(4)
Pages: 729-733
License: View

Authors

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B. K. Burnison

Cite This Article

B. K. Burnison (1980). Modified Dimethyl Sulfoxide (DMSO) Extraction for Chlorophyll Analysis of Phytoplankton. Canadian Journal of Fisheries and Aquatic Sciences, 37(4), 729-733. https://doi.org/10.1139/f80-095

Modified Dimethyl Sulfoxide (DMSO) Extraction for Chlorophyll Analysis of Phytoplankton

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