A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

E. G. Bligh; W. J. Dyer

doi:10.1139/o59-099

journal article Aug 01, 1959

A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

E. G. Bligh W. J. Dyer

Canadian Journal of Biochemistry and Physiology Vol. 37 No. 8 pp. 911-917 · Canadian Science Publishing

View at Publisher Save 10.1139/o59-099

Abstract

Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.

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References

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Details

Published: Aug 01, 1959
Vol/Issue: 37(8)
Pages: 911-917
License: View

Authors

Cite This Article

E. G. Bligh, W. J. Dyer (1959). A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION. Canadian Journal of Biochemistry and Physiology, 37(8), 911-917. https://doi.org/10.1139/o59-099

A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

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