The Effect of Acute Aerobic Exercise on Redox Homeostasis and Mitochondrial Function of Rat White Adipose Tissue

Leonardo Matta; Túlio S. Fonseca; Caroline C. Faria; Niedson Correia Lima-Junior; Dahienne F. De Oliveira; Leonardo Maciel; Luiz F. Boa; Ana Paola T. R. Pierucci; Andrea C. F. Ferreira; José H. M. Nascimento; Denise P. Carvalho; Rodrigo S. Fortunato

doi:10.1155/2021/4593496

journal article Open Access Jan 01, 2021

The Effect of Acute Aerobic Exercise on Redox Homeostasis and Mitochondrial Function of Rat White Adipose Tissue

Túlio S. Fonseca Caroline C. Faria Niedson Correia Lima-Junior Dahienne F. De Oliveira Leonardo Maciel Luiz F. Boa Ana Paola T. R. Pierucci Andrea C. F. Ferreira José H. M. Nascimento Denise P. Carvalho Rodrigo S. Fortunato

Oxidative Medicine and Cellular Longevity Vol. 2021 No. 1 · Wiley

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Abstract

Physical exercise is characterized by an increase in physical and metabolic demand in face of physical stress. It is reported that a single exercise session induces physiological responses through redox signaling to increase cellular function and energy support in diverse organs. However, little is known about the effect of a single bout of exercise on the redox homeostasis and cytoprotective gene expression of white adipose tissue (WAT). Thus, we aimed at evaluating the effects of acute aerobic exercise on WAT redox homeostasis, mitochondrial metabolism, and cytoprotective genic response. Male Wistar rats were submitted to a single moderate‐high running session (treadmill) and were divided into five groups: control (CTRL, without exercise), and euthanized immediately (0 h), 30 min, 1 hour, or 2 hours after the end of the exercise session. NADPH oxidase activity was higher in 0 h and 30 min groups when compared to CTRL group. Extramitochondrial ROS production was higher in 0 h group in comparison to CTRL and 2 h groups. Mitochondrial respiration in phosphorylative state increased in 0 h group when compared to CTRL, 30 min, 1, and 2 h groups. On the other hand, mitochondrial ATP production was lower in 0 h in comparison to 30 min group, increasing in 1 and 2 h groups when compared to CTRL and 0 h groups. CAT activity was lower in all exercised groups when compared to CTRL. Regarding oxidative stress biomarkers, we observed a decrease in reduced thiol content in 0 h group compared to CTRL and 2 h groups, and higher levels of protein carbonylation in 0 and 30 min groups in comparison to the other groups. The levels returned to basal condition in 2 h group. Furthermore, aerobic exercise increased NRF2, GPX2, HMOX1, SOD1, and CAT mRNA levels. Taken together, our results suggest that one session of aerobic exercise can induce a transient prooxidative state in WAT, followed by an increase in antioxidant and cytoprotective gene expression.

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References

67

[1]

Caspersen C. J. "Physical activity, exercise, and physical fitness: definitions and distinctions for health-related research" Public Health Reports (1985)

[2]

10.1016/j.freeradbiomed.2016.02.025

[3]

10.1152/ajpendo.00568.2012

[4]

10.3390/antiox9010057

[5]

10.1113/jp270646

[6]

Oxygen Consumption and Usage During Physical Exercise: The Balance Between Oxidative Stress and ROS-Dependent Adaptive Signaling

Zsolt Radak, Zhongfu Zhao, Erika Koltai et al.

Antioxidants & Redox Signaling 10.1089/ars.2011.4498

[7]

Nrf2 mediates redox adaptations to exercise

Aaron J. Done, Tinna Traustadóttir

Redox Biology 10.1016/j.redox.2016.10.003

[8]

10.1179/1351000214y.0000000105

[9]

10.1016/j.brainres.2019.02.004

[10]

10.1016/j.freeradbiomed.2011.10.440

[11]

10.1016/j.expneurol.2014.09.021

[12]

10.1016/j.lfs.2020.117958

[13]

10.1016/j.lfs.2017.09.007

[14]

10.1007/s10552-014-0354-x

[15]

10.1016/j.exger.2013.05.063

[16]

10.1016/b978-0-12-408134-5.00014-7

[17]

10.1038/nrm3072

[18]

10.1111/j.1469-7793.2001.00283.x

[19]

10.1152/ajpcell.00313.2019

[20]

10.1016/j.bbrc.2008.12.127

[21]

10.1089/rej.2007.0576

[22]

10.1016/j.ijcard.2016.03.071

[23]

10.1677/joe-08-0174

[24]

10.1093/clinchem/18.11.1334

[25]

10.1089/thy.2012.0142

[26]

10.1210/jc.2010-1085

[27]

10.3389/fphar.2020.00545

[28]

10.1016/s0076-6879(78)53044-9

[29]

10.1016/s0076-6879(84)05016-3

[30]

10.1016/s0076-6879(84)05015-1

[31]

Quantification of thiols and disulfides

Jakob R. Winther, Colin Thorpe

Biochimica et Biophysica Acta (BBA) - General Subj... 10.1016/j.bbagen.2013.03.031

[32]

10.3389/fphys.2015.00015

[33]

10.1016/j.bbadis.2017.06.016

[34]

Analyzing real-time PCR data by the comparative CT method

Thomas D Schmittgen, Kenneth J Livak

Nature Protocols 10.1038/nprot.2008.73

[35]

Metabolic adaptation and maladaptation in adipose tissue

Edward T. Chouchani, Shingo Kajimura

Nature Metabolism 10.1038/s42255-018-0021-8

[36]

10.1016/j.tem.2012.06.004

[37]

10.1016/j.redox.2013.07.006

[38]

10.1089/ars.2019.7949

[39]

10.2147/copd.s226623

[40]

10.1016/j.bbi.2016.05.010

[41]

10.2165/00007256-200131130-00001

[42]

10.1002/jcb.29208

[43]

10.1038/s41598-020-60286-x

[44]

10.1016/bs.pmbts.2015.06.016

[45]

10.2337/db15-0227

[46]

10.1128/mcb.24.5.1844-1854.2004

[47]

10.1016/j.cmet.2011.08.007

[48]

10.1083/jcb.200709049

[49]

10.1038/nrc3339

[50]

10.1016/j.freeradbiomed.2010.07.027

Showing 50 of 67 references

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Details

Published: Jan 01, 2021
Vol/Issue: 2021(1)
License: View

Authors

Niedson Correia Lima-Junior

D

Dahienne F. De Oliveira

Ana Paola T. R. Pierucci

A

Andrea C. F. Ferreira

J

José H. M. Nascimento

Funding

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior

Conselho Nacional de Desenvolvimento Cientifico e Tecnologico

Fundação Carlos Chagas Filho de Amparo a Pesquisa do Estado do Rio de Janeiro

Cite This Article

Leonardo Matta, Túlio S. Fonseca, Caroline C. Faria, et al. (2021). The Effect of Acute Aerobic Exercise on Redox Homeostasis and Mitochondrial Function of Rat White Adipose Tissue. Oxidative Medicine and Cellular Longevity, 2021(1). https://doi.org/10.1155/2021/4593496

The Effect of Acute Aerobic Exercise on Redox Homeostasis and Mitochondrial Function of Rat White Adipose Tissue

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