The Fas/Fas-Ligand System is Not Required for Bleomycin-induced Pulmonary Fibrosis in Mice

Kazutetsu Aoshiba; SHUJI YASUI; JUN TAMAOKI; Atsushi Nagai

doi:10.1164/ajrccm.162.2.9907012

journal article Aug 01, 2000

The Fas/Fas-Ligand System is Not Required for Bleomycin-induced Pulmonary Fibrosis in Mice

Kazutetsu Aoshiba SHUJI YASUI JUN TAMAOKI Atsushi Nagai

American Review of Respiratory Disease Vol. 162 No. 2 pp. 695-700 · Oxford University Press (OUP)

View at Publisher Save 10.1164/ajrccm.162.2.9907012

Abstract

Abstract
Recent studies suggest that Fas–Fas-ligand (FasL) interactions play an important role in the development of lung injury and fibrosis. However, evidence to support this concept is still indirect. To determine whether Fas–FasL interaction is required for the development of bleomycin-induced pulmonary fibrosis in mice, we used Fas-deficient (lpr) and FasL-deficient (gld) mice as animal models. After intratracheal instillation of bleomycin, we examined the lungs of mice through bronchoalveolar lavage, histologic studies, DNA nick-end labeling, and hydroxyproline assay. The development of cellular infiltrates, bronchiolar and alveolar epithelial apoptosis, and fibrosis following bleomycin instillation in the lungs in lpr mice and gld mice was similar to their development in wild-type mice. The results of this study show that bleomycin-induced pulmonary fibrosis does not require Fas–FasL interaction, and that epithelial cell apoptosis after bleomycin exposure is mediated by Fas-independent pathways.

Topics

No keywords indexed for this article. Browse by subject →

References

21

[1]

Hagimoto "Apoptosis and expression of Fas/ Fas ligand mRNA in bleomycin-induced pulmonary fibrosis in mice." Am. J. Respir. Cell Mol. Biol. (1997) 10.1165/ajrcmb.16.1.8998084

[2]

Hagimoto "Induction of apoptosis and pulmonary fibrosis in mice in response to ligation of Fas antigen." Am. J. Respir. Cell Mol. Biol. (1997) 10.1165/ajrcmb.17.3.2893

[3]

Kuwano "The involvement of Fas-Fas ligand pathway in fibrosing lung diseases." Am. J. Respir. Cell Mol. Biol. (1999) 10.1165/ajrcmb.20.1.2941

[4]

Adachi "Aberrant trans-cription caused by the insertion of an early transposable element in an intron of the Fas antigen gene lpr mice." Proc. Natl. Acad. Sci. U.S.A. (1993) 10.1073/pnas.90.5.1756

[5]

Watanabe-Fukunaga "Lymphoproliferation disorder in mice explained by defects in Fas antigen that mediates apoptosis." Nature (1992) 10.1038/356314a0

[6]

Takahashi "Generalized lymphoproliferative disease in mice, caused by a point mutation in the Fas ligand." Cell (1992) 10.1016/0092-8674(94)90375-1

[7]

Nagai "Administration of alpha 1-proteinase inhibitor ameliorates bleomycin-induced pulmonary fibrosis in hamsters." Am. Rev. Respir. Dis. (1992) 10.1164/ajrccm/145.3.651

[8]

Fujiwara "Postcolumn fluorometric detection system for liquid chromatographic analysis of amino and imino acids using o-phthalaldehyde/N-acetyl-l-cysteine reagent." Anal. Biochem. (1987) 10.1016/0003-2697(87)90547-1

[9]

Chandler "Morphometric estimates of infiltrative cellular changes during the development of bleomycin-induced pulmonary fibrosis in hamsters." Am. J. Pathol. (1983)

[10]

Bigby "Bleomycin-induced lung injury in the rabbit. Analysis and correlation of bronchoalveolar lavage, morphometrics, and fibroblasts stimulating activity." Am. Rev. Respir. Dis. (1985)

[11]

Nomoto "Apoptosis and Fas/Fas ligand mRNA expression in acute immune complex alveolitis in mice." Eur. Respir. J. (1997) 10.1183/09031936.97.10102351

[12]

Vernooy "Intratracheal instillation of lipopolysaccharaide induces apoptosis in bronchial epithelial cells (abstract)." Am. J. Respir. Crit. Care Med. (1999)

[13]

Kawasaki "Systemic injection of tripeptide inhibits caspase activation and ameliorates apoptosis in lipopolysaccharide-induced acute lung injury in mice (abstract)." Am. J. Respir. Crit. Care Med. (1999)

[14]

Berisha "Inhibition of apoptosis as a strategy for lung protection: caspase inhibitors prevent excitotoxic lung injury (abstract)." Am. J. Respir. Crit. Care Med. (1999)

[15]

Kuwano "Inhalation of caspase inhibitors ameliorates apoptosis and fibrosis in bleomycin-induced pneumopathy in mice (abstract)." Am. J. Respir. Crit. Care Med. (1999)

[16]

Chapman "A Fas pathway to pulmonary fibrosis." J. Clin. Invest. (1999) 10.1172/jci7576

[17]

Abreu-Martin "Divergent induction of apoptosis and IL-8 secretion in HT-29 cells in response to TNF-α and ligation of Fas antigen." J. Immunol. (1995) 10.4049/jimmunol.155.9.4147

[18]

Seino "Chemotactic activity of soluble Fas ligand against phagocytes." J. Immunol. (1998) 10.4049/jimmunol.161.9.4484

[19]

Allison "Transgenic expression of CD95 ligand on islet β cells induces a granulocytic infiltration, but does not confer immune privilege upon islet allografts." Proc. Natl. Acad. Sci. U.S.A. (1997) 10.1073/pnas.94.8.3943

[20]

[21]

Kuwano "Essential roles of the Fas-Fas ligand pathway in the development of pulmonary fibrosis." J. Clin. Invest. (1999) 10.1172/jci5628

Cited By

34

Apoptotic cell death in disease—Current understanding of the NCCD 2023

Ilio Vitale, Federico Pietrocola · 2023

Cell Death & Differentiation

Oxidative Stress in Pulmonary Fibrosis

Eva Otoupalova, Sam Smith · 2020

Comprehensive Physiology

Loss of Fas signaling in fibroblasts impairs homeostatic fibrosis resolution and promotes persistent pulmonary fibrosis

Elizabeth F. Redente, Sangeeta Chakraborty · 2020

JCI Insight

Myofibroblast differentiation and survival in fibrotic disease

Kornelia Kis, Xiaoqiu Liu · 2011

Expert Reviews in Molecular Medicin...

Gene expression analysis reveals matrilysin as a key regulator of pulmonary fibrosis in mice and humans

Fengrong Zuo, Naftali Kaminski · 2002

Proceedings of the National Academy...

Metrics

34

Citations

21

References

Details

Published: Aug 01, 2000
Vol/Issue: 162(2)
Pages: 695-700
License: View

Authors

K

Kazutetsu Aoshiba

First Department of Medicine, Tokyo Women's Medical University, Tokyo, Japan

S

SHUJI YASUI

First Department of Medicine, Tokyo Women's Medical University, Tokyo, Japan

J

JUN TAMAOKI

First Department of Medicine, Tokyo Women's Medical University, Tokyo, Japan

A

Atsushi Nagai

First Department of Medicine, Tokyo Women's Medical University, Tokyo, Japan

Cite This Article

Kazutetsu Aoshiba, SHUJI YASUI, JUN TAMAOKI, et al. (2000). The Fas/Fas-Ligand System is Not Required for Bleomycin-induced Pulmonary Fibrosis in Mice. American Review of Respiratory Disease, 162(2), 695-700. https://doi.org/10.1164/ajrccm.162.2.9907012

The Fas/Fas-Ligand System is Not Required for Bleomycin-induced Pulmonary Fibrosis in Mice

You May Also Like