Differentiation of a Porcine Reproductive and Respiratory Syndrome Virus Vaccine Strain from North American Field Strains by Restriction Fragment Length Polymorphism Analysis of ORF 5

Ronald D. Wesley; William L. Mengeling; Kelly M. Lager; Deborah F. Clouser; John G. Landgraf; Merwin L. Frey

doi:10.1177/104063879801000204

journal article Apr 01, 1998

Differentiation of a Porcine Reproductive and Respiratory Syndrome Virus Vaccine Strain from North American Field Strains by Restriction Fragment Length Polymorphism Analysis of ORF 5

Ronald D. Wesley William L. Mengeling Kelly M. Lager Deborah F. Clouser John G. Landgraf Merwin L. Frey

Journal of Veterinary Diagnostic Investigation Vol. 10 No. 2 pp. 140-144 · SAGE Publications

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Abstract

The suitability of restriction fragment length polymorphism (RFLP) analysis for differentiating a porcine reproductive and respiratory syndrome virus (PRRSV) vaccine strain from other North American field strains was investigated. Open reading frame 5 nucleotide sequence data of the vaccine virus, its parent strain VR-2332, and 22 other strains of PRRSV included in this study indicated that 3 restriction enzyme gel patterns characterize the vaccine virus and the parent strain genotype. The combined 3 RFLP patterns differentiate the vaccine and parent virus from other PRRSV strains. This test will be a valuable tool in epidemiologic studies and will be useful in identifying individual strains in cases of multistrain PRRSV infections.

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Metrics

135

Citations

15

References

Details

Published: Apr 01, 1998
Vol/Issue: 10(2)
Pages: 140-144
License: View

Authors

R

Ronald D. Wesley

Virology Swine Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, PO Box 70, Ames, IA 50010

W

William L. Mengeling

Virology Swine Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, PO Box 70, Ames, IA 50010

K

Kelly M. Lager

Virology Swine Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, PO Box 70, Ames, IA 50010

D

Deborah F. Clouser

Virology Swine Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, PO Box 70, Ames, IA 50010

J

John G. Landgraf

Diagnostic Virology Laboratory, National Veterinary Services Laboratories, USDA, Animal and Plant Health Inspection Service, PO Box 844, Ames, IA 50010

M

Merwin L. Frey

Diagnostic Virology Laboratory, National Veterinary Services Laboratories, USDA, Animal and Plant Health Inspection Service, PO Box 844, Ames, IA 50010

Cite This Article

Ronald D. Wesley, William L. Mengeling, Kelly M. Lager, et al. (1998). Differentiation of a Porcine Reproductive and Respiratory Syndrome Virus Vaccine Strain from North American Field Strains by Restriction Fragment Length Polymorphism Analysis of ORF 5. Journal of Veterinary Diagnostic Investigation, 10(2), 140-144. https://doi.org/10.1177/104063879801000204

Differentiation of a Porcine Reproductive and Respiratory Syndrome Virus Vaccine Strain from North American Field Strains by Restriction Fragment Length Polymorphism Analysis of ORF 5

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