Investigating In Vitro Antibacterial Activities of Medicinal Plants Having Folkloric Repute in Ethiopian Traditional Medicine

Mekonnen Sisay; Negussie Bussa; Tigist Gashaw; Getnet Mengistu

doi:10.1177/2515690x19886276

journal article Jan 01, 2019

Investigating In Vitro Antibacterial Activities of Medicinal Plants Having Folkloric Repute in Ethiopian Traditional Medicine

Mekonnen Sisay

Negussie Bussa Tigist Gashaw Getnet Mengistu

Journal of Evidence-Based Integrative Medicine Vol. 24 · SAGE Publications

View at Publisher Save 10.1177/2515690x19886276

Abstract

Medicinal plants are targeted in the search for new antimicrobial agents. Nowadays, there is an alarmingly increasing antimicrobial resistance to available agents with a very slow development of new antimicrobials. It is, therefore, necessary to extensively search for new agents based on the traditional use of herbal medicines as potential source. The antibacterial activity of 80% methanol extracts of the leaves of Verbena officinalis (Vo-80ME), Myrtus communis (Mc-80ME), and Melilotus elegans (Me-80ME) was tested against 6 bacterial isolates using agar well diffusion technique. In each extract, 3 concentrations of 10, 20, and 40 mg/well were tested for each bacterium. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were also determined. Vo-80ME and Mc-80ME exhibited promising antibacterial activity against Staphylococcus aureus with the highest zone of inhibition being 18.67 and 26.16 mm, respectively at concentration of 40 mg/well. Regarding gram-negative bacteria, Vo-80ME exhibited an appreciable activity against Escherichia coli and Salmonella typhi. Mc-80ME displayed remarkable activity against all isolates including Pseudomonas aeruginosa with the maximum zone of inhibition being 22.83 mm. Me-80ME exhibited better antibacterial activity against E coli, but its secondary metabolites had little or no activity against other gram-negative isolates. The MIC values of Vo-80ME ranged from 0.16 to 4.00 mg/mL. The lowest MIC was observed in Mc-80ME, with the value being 0.032 mg/mL. Mc-80ME had bactericidal activity against all tested bacterial isolates. Mc-80ME showed remarkable zone of inhibitions in all tested bacterial isolates. Besides, Vo-80ME showed good antibacterial activity against S aureus, E coli, and S typhi. Conversely, Me-80ME has shown good activity against E coli only. Generally, M communis L and V officinalis have good MIC and MBC results.

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Details

Published: Jan 01, 2019
Vol/Issue: 24
License: View

Authors

M

Mekonnen Sisay

School of Pharmacy, Haramaya University, Harar, Ethiopia

N

Negussie Bussa

Food Science and Post-harvest Technology, Haramaya University, Dire Dawa, Ethiopia

T

Tigist Gashaw

School of Pharmacy, Haramaya University, Harar, Ethiopia

G

Getnet Mengistu

Department of Pharmacy, Wollo University, Dessie, Amhara, Ethiopia

Funding

Haramaya University

Cite This Article

Mekonnen Sisay, Negussie Bussa, Tigist Gashaw, et al. (2019). Investigating In Vitro Antibacterial Activities of Medicinal Plants Having Folkloric Repute in Ethiopian Traditional Medicine. Journal of Evidence-Based Integrative Medicine, 24. https://doi.org/10.1177/2515690x19886276

Investigating In Vitro Antibacterial Activities of Medicinal Plants Having Folkloric Repute in Ethiopian Traditional Medicine

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