Targeted gene expression as a means of altering cell fates and generating dominant phenotypes

Andrea H. Brand; Norbert Perrimon

doi:10.1242/dev.118.2.401

journal article Jun 01, 1993

Targeted gene expression as a means of altering cell fates and generating dominant phenotypes

Andrea H. Brand Norbert Perrimon

Development Vol. 118 No. 2 pp. 401-415 · The Company of Biologists

View at Publisher Save 10.1242/dev.118.2.401

Abstract

ABSTRACT
We have designed a system for targeted gene expression that allows the selective activation of any cloned gene in a wide variety of tissueand cell-specific patterns. The gene encoding the yeast transcriptional activator GAL4 is inserted randomly into the Drosophila genome to drive GAL4 expression from one of a diverse array of genomic enhancers. It is then possible to introduce a gene containing GAL4 binding sites within its promoter, to activate it in those cells where GAL4 is expressed, and to observe the effect of this directed misexpression on development. We have used GAL4-directed transcription to expand the domain of embryonic expression of the homeobox protein even-skipped. We show that even-skipped represses wingless and transforms cells that would normally secrete naked cuticle into denticle secreting cells. The GAL4 system can thus be used to study regulatory interactions during embryonic development. In adults, targeted expression can be used to generate dominant phenotypes for use in genetic screens. We have directed expression of an activated form of the Dras2 protein, resulting in dominant eye and wing defects that can be used in screens to identify other members of the Dras2 signal transduction pathway.

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References

Details

Published: Jun 01, 1993
Vol/Issue: 118(2)
Pages: 401-415
License: View

Authors

A

Andrea H. Brand

1 Department of Genetics Harvard Medical School, Boston, Massachusetts 02115, USA

N

Norbert Perrimon

1 Department of Genetics Harvard Medical School, Boston, Massachusetts 02115, USA; 2 Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts 02115, USA

Cite This Article

Andrea H. Brand, Norbert Perrimon (1993). Targeted gene expression as a means of altering cell fates and generating dominant phenotypes. Development, 118(2), 401-415. https://doi.org/10.1242/dev.118.2.401

Targeted gene expression as a means of altering cell fates and generating dominant phenotypes

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