Quantification of cytochrome c oxidase and tissue oxygenation using CW-NIRS in a mouse cerebral cortex

Mada Hashem; Ying Wu; Jeff F. Dunn

doi:10.1364/boe.435532

journal article Nov 17, 2021

Quantification of cytochrome c oxidase and tissue oxygenation using CW-NIRS in a mouse cerebral cortex

Mada Hashem Ying Wu

Jeff F. Dunn

Biomedical Optics Express Vol. 12 No. 12 pp. 7632 · Optica Publishing Group

View at Publisher Save 10.1364/boe.435532

Abstract

We provide a protocol for measuring the absolute concentration of the oxidized and reduced state of cytochrome c oxidase (CCO) in the cerebral cortex of mice, using broadband continuous-wave NIRS. The algorithm (NIR-AQUA) allows for absolute quantification of CCO and deoxyhemoglobin. Combined with an anoxia pulse, this also allows for quantification of total hemoglobin, and tissue oxygen saturation. CCO in the cortex was 4.9 ± 0.1 μM (mean ± SD, n=6). In normoxia, 84% of CCO was oxidized. We include hypoxia and cyanide validation studies to show CCO can be quantified independently to hemoglobin. This can be applied to study oxidative metabolism in the many rodent models of neurological disease.

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References

Details

Published: Nov 17, 2021
Vol/Issue: 12(12)
Pages: 7632
License: View

Authors

M

Mada Hashem

University of Calgary

Y

Ying Wu

University of Calgary

J

Jeff F. Dunn

University of Calgary

Funding

National Institutes of Health Award: R21 EB021397

Canada Foundation for Innovation Award: Project 4933

Natural Sciences and Engineering Research Council of Canada Award: RGPIN-2015-06517

Alberta Graduate Excellence Scholarship

Eyes High International Doctoral Scholarship

Cite This Article

Mada Hashem, Ying Wu, Jeff F. Dunn (2021). Quantification of cytochrome c oxidase and tissue oxygenation using CW-NIRS in a mouse cerebral cortex. Biomedical Optics Express, 12(12), 7632. https://doi.org/10.1364/boe.435532

Quantification of cytochrome c oxidase and tissue oxygenation using CW-NIRS in a mouse cerebral cortex

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