Compact multicolor two-photon fluorescence microscopy enabled by tailorable continuum generation from self-phase modulation and dispersive wave generation

Lu-Ting Chou; Shao-Hsuan Wu; Hao-Hsuan Hung; Wei-Zong Lin; Zi-Ping Chen; Anatoly A. Ivanov; Shih-Hsuan Chia

doi:10.1364/oe.470602

journal article Oct 17, 2022

Compact multicolor two-photon fluorescence microscopy enabled by tailorable continuum generation from self-phase modulation and dispersive wave generation

Lu-Ting Chou Shao-Hsuan Wu Hao-Hsuan Hung Wei-Zong Lin Zi-Ping Chen Anatoly A. Ivanov Shih-Hsuan Chia

Optics Express Vol. 30 No. 22 pp. 40315 · Optica Publishing Group

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Abstract

By precisely managing fiber-optic nonlinearity with anomalous dispersion, we have demonstrated the control of generating plural few-optical-cycle pulses based on a 24-MHz Chromium:forsterite laser, allowing multicolor two-photon tissue imaging by wavelength mixing. The formation of high-order soliton and its efficient coupling to dispersive wave generation leads to phase-matched spectral broadening, and we have obtained a broadband continuum ranging from 830 nm to 1200 nm, delivering 5-nJ pulses with a pulse width of 10.5 fs using a piece of large-mode-area fiber. We locate the spectral enhancement at around 920 nm for the two-photon excitation of green fluorophores, and we can easily compress the resulting pulse close to its limited duration without the need for active pulse shaping. To optimize the wavelength mixing for sum-frequency excitation, we have realized the management of the power ratio and group delay between the soliton and dispersive wave by varying the initial pulse energy without additional delay control. We have thus demonstrated simultaneous three-color two-photon tissue imaging with contrast management between different signals. Our source optimization leads to efficient two-photon excitation reaching a 500-µm imaging depth under a low 14-mW illumination power. We believe our source development leads to an efficient and compact approach for driving multicolor two-photon fluorescence microscopy and other ultrafast investigations, such as strong-field-driven applications.

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Citations

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References

Details

Published: Oct 17, 2022
Vol/Issue: 30(22)
Pages: 40315
License: View

Authors

Photochemistry Centre

S

Shih-Hsuan Chia

Funding

Ministry of Science and Technology, Taiwan Award: MOST 111-2636-E-A49-013

Cite This Article

Lu-Ting Chou, Shao-Hsuan Wu, Hao-Hsuan Hung, et al. (2022). Compact multicolor two-photon fluorescence microscopy enabled by tailorable continuum generation from self-phase modulation and dispersive wave generation. Optics Express, 30(22), 40315. https://doi.org/10.1364/oe.470602

Compact multicolor two-photon fluorescence microscopy enabled by tailorable continuum generation from self-phase modulation and dispersive wave generation

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