Eosin Nigrosin staining technique in assessment of sperm vitality in medical laboratories – A snippet from our experience on implementing the staining, interpretation and quality control procedures

Archana Shetty; Sandhyarani Mahadev Kanna

doi:10.18231/j.ijogr.2023.048

journal article Open Access May 15, 2023

Eosin Nigrosin staining technique in assessment of sperm vitality in medical laboratories – A snippet from our experience on implementing the staining, interpretation and quality control procedures

Archana Shetty Sandhyarani Mahadev Kanna

Indian Journal of Obstetrics and Gynecology Research Vol. 10 No. 2 pp. 227-229 · IP Innovative Publication Pvt Ltd

View at Publisher Save 10.18231/j.ijogr.2023.048

Abstract

Eosin Nigrosin staining for assessment of sperm vitality is an essential component of basic semen analysis as it helps differentiate between dead and immotile sperms, and has clinical implications in terms of patient treatment and follow up. This staining technique involves minimal use of reagents and simple procedural steps. Standardization of the same is pertinent to warrant accurate and reproducible results in medical laboratories, even those not specialized in infertility care. We wish to share our hands on experience through various stages in implementing this staining technique from challenges faced to putting quality control processes in place as reference for peers.

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Mortimer D, A technical note on the assessment of human sperm vitality using eosin-nigrosin staining.Reprod Biomed Online 2020;40(6):851-5 10.1016/j.rbmo.2020.03.002

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Details

Published: May 15, 2023
Vol/Issue: 10(2)
Pages: 227-229
License: View

Authors

A

Archana Shetty

D

S

Sandhyarani Mahadev Kanna

Dr. Chandramma Dayananda Sagar Institute of Medical Education and Research, Ramanagara, Karnataka, India

Cite This Article

Archana Shetty, Sandhyarani Mahadev Kanna (2023). Eosin Nigrosin staining technique in assessment of sperm vitality in medical laboratories – A snippet from our experience on implementing the staining, interpretation and quality control procedures. Indian Journal of Obstetrics and Gynecology Research, 10(2), 227-229. https://doi.org/10.18231/j.ijogr.2023.048

Eosin Nigrosin staining technique in assessment of sperm vitality in medical laboratories – A snippet from our experience on implementing the staining, interpretation and quality control procedures

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