journal article
Open Access
Nov 13, 2022
Exploring the Antibiotic Resistance Profile of Clinical Klebsiella pneumoniae Isolates in Portugal
Abstract
While antibiotic resistance is rising to dangerously high levels, resistance mechanisms are spreading globally among diverse bacterial species. The emergence of antibiotic-resistant Klebsiella pneumoniae, mainly due to the production of antibiotic-inactivating enzymes, is currently responsible for most treatment failures, threatening the effectiveness of classes of antibiotics used for decades. This study assessed the presence of genetic determinants of β-lactam resistance in 102 multi-drug resistant (MDR) K. pneumoniae isolates from patients admitted to two central hospitals in northern Portugal from 2010 to 2020. Antimicrobial susceptibility testing revealed a high rate (>90%) of resistance to most β-lactam antibiotics, except for carbapenems and cephamycins, which showed antimicrobial susceptibility rates in the range of 23.5–34.3% and 40.2–68.6%, respectively. A diverse pool of β-lactam resistance genetic determinants, including carbapenemases- (i.e., blaKPC-like and blaOXA-48-like), extended-spectrum β-lactamases (ESBL; i.e., blaTEM-like, blaCTX-M-like and blaSHV-like), and AmpC β-lactamases-coding genes (i.e., blaCMY-2-like and blaDHA-like) were found in most K. pneumoniae isolates. blaKPC-like (72.5%) and ESBL genes (37.3–74.5%) were the most detected, with approximately 80% of K. pneumoniae isolates presenting two or more resistance genes. As the optimal treatment of β-lactamase-producing K. pneumoniae infections remains problematic, the high co-occurrence of multiple β-lactam resistance genes must be seen as a serious warning of the problem of antimicrobial resistance.
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Cited By
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Molecular characterization and genome sequencing of selected highly resistant clinical isolates of Pseudomonas aeruginosa and its association with the clustered regularly interspaced palindromic repeat/Cas system
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Hekmat A. Owaid, Mushtak T.S. Al-Ouqaili · 2025
Metrics
32
Citations
69
References
Details
- Published
- Nov 13, 2022
- Vol/Issue
- 11(11)
- Pages
- 1613
- License
- View
Authors
Funding
FCT
Award: LA/P/0045/2020 (ALiCE)
Portuguese Foundation for Science and Technology (FCT)
Award: LA/P/0045/2020 (ALiCE)
Inov4Agro—Associate Laboratory for Innovation, Capacity Building and Sustainability in Agri-Food Production
Award: LA/P/0045/2020 (ALiCE)
Cite This Article
Ricardo Oliveira, Joana Castro, Sónia Silva, et al. (2022). Exploring the Antibiotic Resistance Profile of Clinical Klebsiella pneumoniae Isolates in Portugal. Antibiotics, 11(11), 1613. https://doi.org/10.3390/antibiotics11111613
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