Antioxidant-Rich Extracts of Terminalia ferdinandiana Interfere with Estimation of Cell Viability

Saleha Akter; Rama Addepalli; Michael E. Netzel; Ujang Tinggi; Mary T. Fletcher; Yasmina Sultanbawa; Simone A. Osborne

doi:10.3390/antiox8060191

journal article Open Access Jun 22, 2019

Antioxidant-Rich Extracts of Terminalia ferdinandiana Interfere with Estimation of Cell Viability

Saleha Akter

Rama Addepalli Michael E. Netzel

Ujang Tinggi Mary T. Fletcher

Yasmina Sultanbawa Simone A. Osborne

Antioxidants Vol. 8 No. 6 pp. 191 · MDPI AG

View at Publisher Save 10.3390/antiox8060191

Abstract

The impact of plant extracts and phytochemicals on in vitro cell viability is usually assessed by employing cell viability assays dependent upon the activity of dehydrogenase enzymes. The CellTiter 96® AQueous One Solution Cell Proliferation Assay (CellTiter) was used to measure cell viability in response to antioxidant-rich extracts of Terminalia ferdinandiana fruits. Conflicting results were obtained from this assay whereby higher concentrations of extracts significantly increased cell viability compared to lower concentrations. Intrinsic reductive potential was observed in a cell-free system when extracts were added directly to the CellTiter assay reagent. To confirm this effect in a similar cell proliferation assay, we employed the CellTiter-Blue® Cell Viability Assay and again observed increased viability with increased concentrations of the extracts and direct reduction of the assay reagent by the extracts in cell-free systems. In the search for a cell proliferation assay that would not be directly affected by the plant extracts, we identified the CyQUANT® NF Cell Proliferation Assay that is based on the estimation of DNA content in viable cells. Cell viability decreased with increasing concentrations of the extracts. Accordingly, the results of the present study indicated that cell viability assays reliant upon dehydrogenase activity may lead to false positive results when testing antioxidant-rich plant extracts with intrinsic reductive potential, and alternative cell viability assays should be used to measure the cell viability.

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Details

Published: Jun 22, 2019
Vol/Issue: 8(6)
Pages: 191
License: View

Authors

S

Saleha Akter

Queensland Alliance for Agriculture and Food Innovation (QAAFI), The University of Queensland, Health and Food Sciences Precinct, 39 Kessels Rd, Coopers Plains, QLD 4108, Australia; CSIRO Agriculture and Food, 306 Carmody Road, St Lucia, QLD 4067, Australia

R

Rama Addepalli

CSIRO Agriculture and Food, 306 Carmody Road, St Lucia, QLD 4067, Australia

M

Michael E. Netzel

Queensland Alliance for Agriculture and Food Innovation (QAAFI), The University of Queensland, Health and Food Sciences Precinct, 39 Kessels Rd, Coopers Plains, QLD 4108, Australia

U

Ujang Tinggi

Queensland Health Forensic and Scientific Services, Health and Food Sciences Precinct, 39 Kessels Rd, Coopers Plains, Qld 4108, Australia

M

Mary T. Fletcher

Queensland Alliance for Agriculture and Food Innovation (QAAFI), The University of Queensland, Health and Food Sciences Precinct, 39 Kessels Rd, Coopers Plains, QLD 4108, Australia

Y

Yasmina Sultanbawa

Queensland Alliance for Agriculture and Food Innovation (QAAFI), The University of Queensland, Health and Food Sciences Precinct, 39 Kessels Rd, Coopers Plains, QLD 4108, Australia

S

Simone A. Osborne

CSIRO Agriculture and Food, 306 Carmody Road, St Lucia, QLD 4067, Australia

Funding

Rural Industries Research and Development Corporation Award: 201430161

Cite This Article

Saleha Akter, Rama Addepalli, Michael E. Netzel, et al. (2019). Antioxidant-Rich Extracts of Terminalia ferdinandiana Interfere with Estimation of Cell Viability. Antioxidants, 8(6), 191. https://doi.org/10.3390/antiox8060191

Antioxidant-Rich Extracts of Terminalia ferdinandiana Interfere with Estimation of Cell Viability

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