journal article Open Access Jun 09, 2020

Carotenoids and Chlorophylls as Antioxidants

Antioxidants Vol. 9 No. 6 pp. 505 · MDPI AG
View at Publisher Save 10.3390/antiox9060505
Abstract
Chlorophylls and carotenoids are natural pigments that are present in our daily diet, especially with the increasing tendency towards more natural and healthy behaviors among consumers. As disturbed antioxidant homeostasis capacities seem to be implicated in the progress of different pathologies, the antioxidant properties of both groups of lipophilic compounds have been studied. The objective of this review was to analyze the state-of-the-art advances in this field. We conducted a systematic bibliographic search (Web of Science™ and Scopus®), followed by a comprehensive and critical description of the results, with special emphasis on highly cited and more recently published research. In addition to an evaluative description of the methodologies, this review discussed different approaches used to obtain a physiological perspective, from in vitro studies to in vivo assays using oxidative biomarkers. From a chemical viewpoint, many studies have demonstrated how a pigment’s structure influences its antioxidant response and the underlying mechanisms. The major outcome is that this knowledge is essential for interpreting new data in a metabolic networks context in the search for more direct applications to health. A promising era is coming where the term “antioxidant” is understood in terms of its broadest significance.
Topics

No keywords indexed for this article. Browse by subject →

References
206
[1]
Apak, R., Capanoglu, E., and Shahidi, F. (2018). Nomenclature and general classification of antioxidant activity/capacity assays. Measurement of Antioxidant Activity & Capacity: Recent Trends and Applications, John Wiley & Sons Ltd.. Chapter 1. 10.1002/9781119135388
[2]
Apak, R., Capanoglu, E., and Shahidi, F. (2018). Evaluation of antioxidant activity/capacity measurement methods for food products. Measurement of Antioxidant Activity & Capacity: Recent Trends and Applications, John Wiley & Sons Ltd.. Chapter 13. 10.1002/9781119135388
[3]
Halliwell "How to Characterize a Biological Antioxidant" Free Radic. Res. Commun. (1990) 10.3109/10715769009148569
[4]
Apak "Antioxidant Activity/Capacity Measurement. 1. Classification, Physicochemical Principles, Mechanisms, and Electron Transfer (ET)-Based Assays" J. Agric. Food Chem. (2016) 10.1021/acs.jafc.5b04739
[5]
Pellegrini, N., Vitaglione, P., Granato, D., and Fogliano, V. (2018). Twenty-five years of total antioxidant capacity measurement of foods and biological fluids: Merits and limitations. J. Sci. Food Agric. 10.1002/jsfa.9550
[6]
Munialo "Critical evaluation of the extrapolation of data relative to antioxidant function from the laboratory and their implications on food production and human health: A review" Int. J. Food Sci. Technol. (2019) 10.1111/ijfs.14135
[7]
Cao "Oxygen-radical absorbance capacity assay for antioxidants" Free Rad. Biol. Med. (1993) 10.1016/0891-5849(93)90027-r
[8]
Cao "Measurement of oxygen radical absorbance capacity in biological samples" Methods Enzymol. (1999) 10.1016/s0076-6879(99)99008-0
[9]
The problems of using one-dimensional methods to evaluate multifunctional food and biological antioxidants

Edwin N Frankel, Anne S Meyer

Journal of the Science of Food and Agriculture 2000 10.1002/1097-0010(200010)80:13<1925::aid-jsfa714>3.0.co;2-4
[10]
Karadag "Review of Methods to Determine Antioxidant Capacities" Food Anal. Methods (2009) 10.1007/s12161-008-9067-7
[11]
Ou "Development and Validation of an Improved Oxygen Radical Absorbance Capacity Assay Using Fluorescein as the Fluorescent Probe" J. Agric. Food Chem. (2001) 10.1021/jf010586o
[12]
Prior "Assays for hydrophilic and lipophilic antioxidant capacity (oxygen radical absorbance capacity (ORACFL)) of plasma and other biological and food samples" J. Agric. Food Chem. (2003) 10.1021/jf0262256
[13]
Anderson "Protective action of carotenoid pigments against photodynamic damage to liposomes" Photochem. Photobiol. (1973) 10.1111/j.1751-1097.1973.tb06440.x
[14]
Krinsky "Interaction of oxygen and oxy-radicals with carotenoids" J. Natl. Cancer Inst. (1982)
[15]
Miki "Biological functions and activities of animal carotenoids" Pure App. Chem. (1991) 10.1351/pac199163010141
[16]
Hill "Exogenous retinoic acid causes specific alterations in the development of the midbrain and hindbrain of the zebrafish embryo including positional respecification of the Mauthner neuron" Mech. Dev. (1995) 10.1016/0925-4773(94)00321-d
[17]
Rengel "Exogenously incorporated ketocarotenoids in large unilamellar vesicles. Protective activity against peroxidation" Biochim. Biophys. Acta (2000) 10.1016/s0005-2736(99)00194-7
[18]
Matsushita "Antioxidant activity of polar carotenoids including astaxanthin-β-glucoside from marine bacterium on PC liposomes" Fisheries Sci. (2000) 10.1046/j.1444-2906.2000.00155.x
[19]
"Investigation into the potential chemical mechanism of carotenoids pro-oxidant activity with liposomes under UV-irradiation" J. Serb. Chem. Soc. (2017) 10.2298/jsc170224078c
[20]
Simic, M.G., and Karel, M. (1980). Recent trends in food applications of antioxidants. Autoxidation in Food and Biological Systems, Plenum Press. 10.1007/978-1-4757-9351-2
[21]
Frankel "Interfacial phenomena in the evaluation of antioxidants: Bulk oils vs emulsion" J. Agric. Food Chem. (1994) 10.1021/jf00041a001
[22]
Laguerre "What makes good antioxidants in lipid-based systems? The next theories beyond the polar paradox" Crit. Rev. Food Sci. Nutr. (2015) 10.1080/10408398.2011.650335
[23]
Kevers "Evolution of Antioxidant Capacity during Storage of Selected Fruits and Vegetables" J. Agric. Food Chem. (2007) 10.1021/jf071736j
[24]
Wayner "Quantitative measurement of the total, peroxyl radical-trapping antioxidant capability of human blood plasma by controlled peroxidation: The important contribution made by plasma proteins" FEBS Lett. (1985) 10.1016/0014-5793(85)81208-4
[25]
Miller "[241 Total antioxidant status in plasma and body fluids" Methods in Enzymology (1994) 10.1016/0076-6879(94)34095-1
[26]
Antioxidant Activity of β-Carotene Compounds in Different in Vitro Assays

Lars Mueller, Volker Boehm

Molecules 2011 10.3390/molecules16021055
[27]
Wright "Predicting the activity of phenolic antioxidants: Theoretical method, analysis of substituent effects, and application to major families of antioxidants" J. Am. Chem. Soc. (2001) 10.1021/ja002455u
[28]
The Ferric Reducing Ability of Plasma (FRAP) as a Measure of “Antioxidant Power”: The FRAP Assay

Iris F.F. Benzie, J.J. Strain

Analytical Biochemistry 1996 10.1006/abio.1996.0292
[29]
Benzie "Ferric reducing/antioxidant power assay: Direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration" Methods Enzymol. (1999) 10.1016/s0076-6879(99)99005-5
[30]
Halvorsen "A Systematic Screening of Total Antioxidants in Dietary Plants" Nutr. J. (2002) 10.1093/jn/132.3.461
[31]
Payne "Antioxidant assays—Consistent findings from FRAP and ORAC reveal a negative impact of organic cultivation on antioxidant potential in spinach but not watercress or rocket leaves" Food Sci. Nutr. (2013) 10.1002/fsn3.71
[32]
Apak "Novel Total Antioxidant Capacity Index for Dietary Polyphenols and Vitamins C and E, Using Their Cupric Ion Reducing Capability in the Presence of Neocuproine: CUPRAC Method" J. Agric. Food Chem. (2004) 10.1021/jf048741x
[33]
Serafini "Understanding the association between dietary antioxidants, redox status and disease: Is the Total Antioxidant Capacity the right tool?" Redox Rep. (2004) 10.1179/135100004225004814
[34]
Apak "Comparative Evaluation of Various Total Antioxidant Capacity Assays Applied to Phenolic Compounds with the CUPRAC Assay" Molecules (2007) 10.3390/12071496
[35]
Prior "Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements" J. Agric. Food Chem. (2005) 10.1021/jf0502698
[36]
Nilsson "Comparison of the 2,2′-azinobis-3-ethylbenzotiazo-line-6-sulfonic acid (ABTS) and ferric reducing anti-oxidant power (FRAP) methods to asses the total antioxidant capacity in extracts of fruit and vegetables" Mol. Nutr. Food Res. (2005) 10.1002/mnfr.200400083
[37]
Spectrophotometric and Chromatographic Assessment of Contributions of Carotenoids and Chlorophylls to the Total Antioxidant Capacities of Plant Foods

Kevser Sözgen Başkan, Esma Tütem, Nihat Özer et al.

Journal of Agricultural and Food Chemistry 2013 10.1021/jf403356h
[38]
Shalaby "Comparison of DPPH and ABTS assays for determining antioxidant potential of water and methanol extracts of Spirulina platensis" Indian J. Mar. Sci. (2013)
[39]
Pulido "Antioxidant activity of fresh and processed edible seaweeds: Antioxidant activity of seaweeds" J. Sci. Food Agric. (2001) 10.1002/jsfa.842
[40]
Arnao "Some methodological problems in the determination of antioxidant activity using chromogen radicals: A practical case" Trends Food Sci. Technol. (2000) 10.1016/s0924-2244(01)00027-9
[41]
Ferruzzi "Trolox Equivalent Antioxidant Capacity of Different Geometrical Isomers of α-Carotene, β-Carotene, Lycopene, and Zeaxanthin" J. Agric. Food Chem. (2002) 10.1021/jf010888q
[42]
Awika "Screening Methods to Measure Antioxidant Activity of Sorghum (Sorghum bicolor) and Sorghum Products" J. Agric. Food Chem. (2003) 10.1021/jf034790i
[43]
The hydrophilic and lipophilic contribution to total antioxidant activity

Marino B. Arnao, Antonio Cano, Manuel Acosta

Food Chemistry 2001 10.1016/s0308-8146(00)00324-1
[44]
Antioxidant Determinations by the Use of a Stable Free Radical

MARSDEN S. BLOIS

Nature 1958 10.1038/1811199a0
[45]
Use of a free radical method to evaluate antioxidant activity

W. Brand-Williams, M.E. Cuvelier, C. Berset

LWT 1995 10.1016/s0023-6438(95)80008-5
[46]
Plank "Determination of Antioxidant Activity in Foods and Beverages by Reaction with 2,2′-Diphenyl-1-Picrylhydrazyl (DPPH): Collaborative Study First Action 2012.04" J. AOAC Int. (2012) 10.5740/jaoacint.cs2012_04
[47]
Antioxidant activity applying an improved ABTS radical cation decolorization assay

Roberta Re, Nicoletta Pellegrini, Anna Proteggente et al.

Free Radical Biology and Medicine 1999 10.1016/s0891-5849(98)00315-3
[48]
Roginsky "Review of methods to determine chain-breaking antioxidant activity in food" Food Chem. (2005) 10.1016/j.foodchem.2004.08.004
[49]
Ghiselli "A fluorescence-based method for measuring total plasma antioxidant capability" Free Radic. Biol. Med. (1995) 10.1016/0891-5849(94)00102-p
[50]
Miller "A Novel Method for Measuring Antioxidant Capacity and its Application to Monitoring the Antioxidant Status in Premature Neonates" Clin. Sci. (1993) 10.1042/cs0840407

Showing 50 of 206 references

Metrics
431
Citations
206
References
Details
Published
Jun 09, 2020
Vol/Issue
9(6)
Pages
505
License
View
Funding
Ministerio de Ciencia, Investigación y Universidades Award: RTI2018-095415-B-I00
Cite This Article
Antonio Pérez-Gálvez, Isabel Viera, Maria Roca (2020). Carotenoids and Chlorophylls as Antioxidants. Antioxidants, 9(6), 505. https://doi.org/10.3390/antiox9060505