Deposition of Cell Culture Coatings Using a Cold Plasma Deposition Method

Denis O’Sullivan; Hazel McArdle; Sing Wei Ng; Paula Bourke; Robert Forster; Liam O’Neill

doi:10.3390/app10196670

journal article Open Access Sep 24, 2020

Deposition of Cell Culture Coatings Using a Cold Plasma Deposition Method

Denis O’Sullivan

Hazel McArdle Sing Wei Ng Paula Bourke

Robert Forster Liam O’Neill

Applied Sciences Vol. 10 No. 19 pp. 6670 · MDPI AG

View at Publisher Save 10.3390/app10196670

Abstract

Collagen coatings were applied onto polystyrene microplates using a cold atmospheric pressure plasma process. The coatings were compared to standard wet chemical collagen thin films using microscopy, surface energy, infra-red spectroscopy, electrophoresis, and cell culture techniques. Thin films were also deposited on gold electrodes using both coating methods and their structural and barrier properties probed using cyclic voltammetry. While the wet chemical technique produced a thicker deposit, both films appear equivalent in terms of coverage, porosity, structure, and chemistry. Significantly, the cold plasma method preserves both the primary and secondary structure of the protein and this results in high biocompatibility and cell activity that is at least equivalent to the standard wet chemical technique. The significance of these results is discussed in relation to the benefits of a single step plasma coating in comparison to the traditional multi-step aseptic coating technique.

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Citations

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References

Details

Published: Sep 24, 2020
Vol/Issue: 10(19)
Pages: 6670
License: View

Authors

D

Denis O’Sullivan

TheraDep, Questum, Ballingarrane Science and Technology Park, Co. Tipperary, Clonmel, Ireland; School of Biosystems and Food Engineering, University College Dublin, Belfield, Dublin D4, Ireland

H

Hazel McArdle

School of Chemical Sciences, FutureNeuro SFI Research Centre, National Centre for Centre Research, Dublin City University, Dublin D9, Ireland

S

Sing Wei Ng

School of Biosystems and Food Engineering, University College Dublin, Belfield, Dublin D4, Ireland

P

Paula Bourke

School of Biosystems and Food Engineering, University College Dublin, Belfield, Dublin D4, Ireland

R

Robert Forster

School of Chemical Sciences, FutureNeuro SFI Research Centre, National Centre for Centre Research, Dublin City University, Dublin D9, Ireland

L

Liam O’Neill

TheraDep, Questum, Ballingarrane Science and Technology Park, Co. Tipperary, Clonmel, Ireland

Funding

Science Foundation Ireland Award: 16/RC/3948

Irish Research Council Award: EBPPG/2018/82

Cite This Article

Denis O’Sullivan, Hazel McArdle, Sing Wei Ng, et al. (2020). Deposition of Cell Culture Coatings Using a Cold Plasma Deposition Method. Applied Sciences, 10(19), 6670. https://doi.org/10.3390/app10196670

Deposition of Cell Culture Coatings Using a Cold Plasma Deposition Method

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