A Highly Sensitive Urinary Exosomal miRNAs Biosensor Applied to Evaluation of Prostate Cancer Progression

Yueh-Er Chiou; Kai-Jie Yu; Sow-Neng Pang; Yan-Lin Yang; See-Tong Pang; Wen-Hui Weng

doi:10.3390/bioengineering9120803

journal article Open Access Dec 14, 2022

A Highly Sensitive Urinary Exosomal miRNAs Biosensor Applied to Evaluation of Prostate Cancer Progression

Yueh-Er Chiou Kai-Jie Yu

Sow-Neng Pang Yan-Lin Yang See-Tong Pang

Wen-Hui Weng

Bioengineering Vol. 9 No. 12 pp. 803 · MDPI AG

View at Publisher Save 10.3390/bioengineering9120803

Abstract

Prostate cancer is the most common cancer in the male population, carrying a significant disease burden. PSA is a widely available screening tools for this disease. Current screen-printed carbon electrode (SPCE)-based biosensors use a two-pronged probe approach to capture urinary miRNA. We were able to successfully detect specific exosomal miRNAs (exomiRs) in the urine of patients with prostate cancer, including exomiR-451 and exomiR-21, and used electrochemistry for measurement and analysis. Our results significantly reaffirmed the presence of exomiR-451 in urine and that a CV value higher than 220 nA is capable of identifying the presence of disease (p-value = 0.005). Similar results were further proven by a PAS greater than 4 (p-value = 0.001). Moreover, a higher urinary exomiR-21 was observed in the high-T3b stage; this significantly decreased following tumor removal (p-values were 0.016 and 0.907, respectively). According to analysis of the correlation with tumor metastasis, a higher exomiR-21 was associated with lymphatic metastasis (p-value 0.042), and higher exomiR-461 expression was correlated with tumor stage (p-value 0.031), demonstrating that the present exomiR biosensor can usefully predict tumor progression. In conclusion, this biosensor represents an easy-to-use, non-invasive screening tool that is both sensitive and specific. We strongly believe that this can be used in conjunction with PSA for the screening of prostate cancer.

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Details

Published: Dec 14, 2022
Vol/Issue: 9(12)
Pages: 803
License: View

Authors

Y

Yueh-Er Chiou

Department of Nursing, College of Medicine, Fu Jen Catholic University, New Taipei City 242, Taiwan

K

Kai-Jie Yu

Department of Chemical Engineering and Biotechnology and Graduate Institute of Biochemical and Biomedical Engineering, National Taipei University of Technology, Taipei City 106, Taiwan; Division of Urology, Department of Surgery, Chang Gung Memorial Hospital, Taoyuan 33305, Taiwan

S

Sow-Neng Pang

Department of Emergency Medicine, Monash Medical Centre, Melbourne, VIC 3083, Australia

Y

Yan-Lin Yang

Department of Chemical Engineering and Biotechnology and Graduate Institute of Biochemical and Biomedical Engineering, National Taipei University of Technology, Taipei City 106, Taiwan

S

See-Tong Pang

Division of Urology, Department of Surgery, Chang Gung Memorial Hospital, Taoyuan 33305, Taiwan

W

Wen-Hui Weng

Department of Chemical Engineering and Biotechnology and Graduate Institute of Biochemical and Biomedical Engineering, National Taipei University of Technology, Taipei City 106, Taiwan

Funding

Ministry of Science and Technology Award: MOST 110-2637-E-027-003-

National Taipei University of Technology Award: NTUT-CGMH-109-03

Cite This Article

Yueh-Er Chiou, Kai-Jie Yu, Sow-Neng Pang, et al. (2022). A Highly Sensitive Urinary Exosomal miRNAs Biosensor Applied to Evaluation of Prostate Cancer Progression. Bioengineering, 9(12), 803. https://doi.org/10.3390/bioengineering9120803

A Highly Sensitive Urinary Exosomal miRNAs Biosensor Applied to Evaluation of Prostate Cancer Progression

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