DNA-Binding Protein Dps Protects Escherichia coli Cells against Multiple Stresses during Desiccation

Nataliya Loiko; Ksenia Tereshkina; Vladislav Kovalenko; Andrey Moiseenko; Eduard Tereshkin; Olga S. Sokolova; Yurii Krupyanskii

doi:10.3390/biology12060853

journal article Open Access Jun 14, 2023

DNA-Binding Protein Dps Protects Escherichia coli Cells against Multiple Stresses during Desiccation

Nataliya Loiko

Ksenia Tereshkina Vladislav Kovalenko Andrey Moiseenko Eduard Tereshkin Olga S. Sokolova

Yurii Krupyanskii

Biology Vol. 12 No. 6 pp. 853 · MDPI AG

View at Publisher Save 10.3390/biology12060853

Abstract

Gradual dehydration is one of the frequent lethal yet poorly understood stresses that bacterial cells constantly face in the environment when their micro ecotopes dry out, as well as in industrial processes. Bacteria successfully survive extreme desiccation through complex rearrangements at the structural, physiological, and molecular levels, in which proteins are involved. The DNA-binding protein Dps has previously been shown to protect bacterial cells from many adverse effects. In our work, using engineered genetic models of E. coli to produce bacterial cells with overproduction of Dps protein, the protective function of Dps protein under multiple desiccation stresses was demonstrated for the first time. It was shown that the titer of viable cells after rehydration in the experimental variants with Dps protein overexpression was 1.5–8.5 times higher. Scanning electron microscopy was used to show a change in cell morphology upon rehydration. It was also proved that immobilization in the extracellular matrix, which is greater when the Dps protein is overexpressed, helps the cells survive. Transmission electron microscopy revealed disruption of the crystal structure of DNA–Dps crystals in E. coli cells that underwent desiccation stress and subsequent watering. Coarse-grained molecular dynamics simulations showed the protective function of Dps in DNA–Dps co-crystals during desiccation. The data obtained are important for improving biotechnological processes in which bacterial cells undergo desiccation.

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Details

Published: Jun 14, 2023
Vol/Issue: 12(6)
Pages: 853
License: View

Authors

N

Nataliya Loiko

Winogradsky Institute of Microbiology, Fundamentals of Biotechnology Federal Research Center, Russian Academy of Sciences, 117312 Moscow, Russia

K

Ksenia Tereshkina

Semenov Federal Research Center for Chemical Physics, Russian Academy of Sciences, 119991 Moscow, Russia

V

Vladislav Kovalenko

Semenov Federal Research Center for Chemical Physics, Russian Academy of Sciences, 119991 Moscow, Russia

A

Andrey Moiseenko

Semenov Federal Research Center for Chemical Physics, Russian Academy of Sciences, 119991 Moscow, Russia; Faculty of Biology, Lomonosov Moscow State University, 119234 Moscow, Russia

E

Eduard Tereshkin

Semenov Federal Research Center for Chemical Physics, Russian Academy of Sciences, 119991 Moscow, Russia

O

Olga S. Sokolova

Faculty of Biology, Lomonosov Moscow State University, 119234 Moscow, Russia

Y

Yurii Krupyanskii

Semenov Federal Research Center for Chemical Physics, Russian Academy of Sciences, 119991 Moscow, Russia

Funding

RSF Award: 23-24-00250

Cite This Article

Nataliya Loiko, Ksenia Tereshkina, Vladislav Kovalenko, et al. (2023). DNA-Binding Protein Dps Protects Escherichia coli Cells against Multiple Stresses during Desiccation. Biology, 12(6), 853. https://doi.org/10.3390/biology12060853

DNA-Binding Protein Dps Protects Escherichia coli Cells against Multiple Stresses during Desiccation

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