Chitosan-Modified AgNPs Efficiently Inhibit Swine Coronavirus-Induced Host Cell Infections via Targeting the Spike Protein

Dongliang Wang; Caiyun Yin; Yihan Bai; Mingxia Zhou; Naidong Wang; Chunyi Tong; Yi Yang; Bin Liu

doi:10.3390/biom14091152

journal article Open Access Sep 13, 2024

Chitosan-Modified AgNPs Efficiently Inhibit Swine Coronavirus-Induced Host Cell Infections via Targeting the Spike Protein

Dongliang Wang

Caiyun Yin Yihan Bai Mingxia Zhou Naidong Wang Chunyi Tong Yi Yang

Bin Liu

Biomolecules Vol. 14 No. 9 pp. 1152 · MDPI AG

View at Publisher Save 10.3390/biom14091152

Abstract

The COVID-19 pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has filled a gap in our knowledge regarding the prevention of CoVs. Swine coronavirus (CoV) is a significant pathogen that causes huge economic losses to the global swine industry. Until now, anti-CoV prevention and control have been challenging due to the rapidly generated variants. Silver nanoparticles (AgNPs) with excellent antimicrobial activity have attracted great interest for biosafety prevention and control applications. In this study, we synthesized chitosan-modified AgNPs (Chi-AgNPs) with good biocompatibility to investigate their antiviral effects on swine CoVs. In vitro assays showed that Chi-AgNPs could significantly impaired viral entry. The direct interaction between Chi-AgNPs and CoVs can destroy the viral surface spike (S) protein secondary structure associated with viral membrane fusion, which is caused by the cleavage of disulfide bonds in the S protein. Moreover, the mechanism showed that Chi-AgNPs reduced the virus-induced apoptosis of Vero cells via the ROS/p53 signaling activation pathway. Our data suggest that Chi-AgNPs can serve as a preventive strategy for CoVs infection and provide a molecular basis for the viricidal effect of Chi-AgNPs on CoVs.

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References

Details

Published: Sep 13, 2024
Vol/Issue: 14(9)
Pages: 1152
License: View

Authors

D

Dongliang Wang

College of Biology, Hunan University, Changsha 410082, China; Hunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China

C

Caiyun Yin

College of Biology, Hunan University, Changsha 410082, China

Y

Yihan Bai

Hunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China

M

Mingxia Zhou

Hunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China

N

Naidong Wang

Hunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China

C

Chunyi Tong

College of Biology, Hunan University, Changsha 410082, China

Y

Yi Yang

Hunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China

B

Bin Liu

College of Biology, Hunan University, Changsha 410082, China

Funding

National Natural Science Foundation of China Award: 2023M741130

China Postdoctoral Science Foundation Award: 2023M741130

Hunan Provincial Natural Science Foundation of China Award: 2023M741130

Hunan Province Technology Breakthrough Project Award: 2023M741130

Cite This Article

Dongliang Wang, Caiyun Yin, Yihan Bai, et al. (2024). Chitosan-Modified AgNPs Efficiently Inhibit Swine Coronavirus-Induced Host Cell Infections via Targeting the Spike Protein. Biomolecules, 14(9), 1152. https://doi.org/10.3390/biom14091152

Chitosan-Modified AgNPs Efficiently Inhibit Swine Coronavirus-Induced Host Cell Infections via Targeting the Spike Protein

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