journal article
Open Access
Jun 10, 2021
Bioengineering Approaches to Improve In Vitro Performance of Prepubertal Lamb Oocytes
Abstract
Juvenile in vitro embryo technology (JIVET) provides exciting opportunities in animal reproduction by reducing the generation intervals. Prepubertal oocytes are also relevant models for studies on oncofertility. However, current JIVET efficiency is still unpredictable, and further improvements are needed in order for it to be used on a large-scale level. This study applied bioengineering approaches to recreate: (1) the three-dimensional (3D) structure of the cumulus–oocyte complex (COC), by constructing—via bioprinting technologies—alginate-based microbeads (COC-microbeads) for 3D in vitro maturation (3D-IVM); (2) dynamic IVM conditions, by culturing the COC in a millifluidic bioreactor; and (3) an artificial follicular wall with basal membrane, by adding granulosa cells (GCs) and type I collagen (CI) during bioprinting. The results show that oocyte nuclear and cytoplasmic maturation, as well as blastocyst quality, were improved after 3D-IVM compared to 2D controls. The dynamic 3D-IVM did not enhance oocyte maturation, but it improved oocyte bioenergetics compared with static 3D-IVM. The computational model showed higher oxygen levels in the bioreactor with respect to the static well. Microbead enrichment with GCs and CI improved oocyte maturation and bioenergetics. In conclusion, this study demonstrated that bioengineering approaches that mimic the physiological follicle structure could be valuable tools to improve IVM and JIVET.
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Details
- Published
- Jun 10, 2021
- Vol/Issue
- 10(6)
- Pages
- 1458
- License
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Authors
Funding
Project “Demetra” (Dipartimenti di Eccellenza 2018- 2022, CUP code: C46C18000530001), funded by the Italian Ministry for Education, University and Research
Award: Research Grant n˚ VERI00086
UE - FSE-FSER, PON RI 2014-2020 Action I.1 - "Innovative doctoral of industrial interest" - a.a. 2016/2017, XXXII cycle, PhD Program "Functional and Applied Genomics and Proteomics
Award: DOT1302781" - Grant n°2
Cite This Article
Antonella Mastrorocco, Ludovica Cacopardo, Daniela Lamanna, et al. (2021). Bioengineering Approaches to Improve In Vitro Performance of Prepubertal Lamb Oocytes. Cells, 10(6), 1458. https://doi.org/10.3390/cells10061458
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