Nebivolol Increases Nitric Oxide Synthase via β3 Adrenergic Receptor in Endothelial Cells Following Exposure to Plasma from Preeclamptic Patients

Thaina Omia Bueno-Pereira; Priscila Rezeck Nunes; Mariana Bertozzi Matheus; Ananda Lini Vieira da Rocha; Valéria Cristina Sandrim

doi:10.3390/cells11050883

journal article Open Access Mar 04, 2022

Nebivolol Increases Nitric Oxide Synthase via β3 Adrenergic Receptor in Endothelial Cells Following Exposure to Plasma from Preeclamptic Patients

Thaina Omia Bueno-Pereira

Priscila Rezeck Nunes

Mariana Bertozzi Matheus

Ananda Lini Vieira da Rocha Valéria Cristina Sandrim

Cells Vol. 11 No. 5 pp. 883 · MDPI AG

View at Publisher Save 10.3390/cells11050883

Abstract

Background: Low bioavailability of nitric oxide (NO) is related to the pathophysiology of preeclampsia (PE). In the present study, we investigated the effect of nebivolol (NEB), a β3-receptor agonist with vasodilator properties, on the NO synthesis in endothelial cells incubated with plasma from preeclamptic patients. Methods and results: Human umbilical vein endothelial cells (HUVECs) were incubated with plasma from healthy pregnant (HP) and PE women; NO quantification was assessed by a fluorescence compound. We found that endothelial cells incubated with plasma from women with PE show lower NO levels compared with the HP group (p < 0.0001). However, NEB treatment increases NO levels, partially, mediated by β3 adrenergic receptors (p < 0.0001) and through eNOS activation (p < 0.0001). Conclusions: Our results suggest that NEB acts in NO synthesis through eNOS activation and β3 adrenergic receptors in the endothelium. However, further studies will be needed to understand this molecule.

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Details

Published: Mar 04, 2022
Vol/Issue: 11(5)
Pages: 883
License: View

Authors

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Thaina Omia Bueno-Pereira