Collectible Cell Lines of Larix sibirica Obtained by Somatic Embryogenesis and Their Ability to Regenerate

Iraida N. Tretyakova; Maria E. Park

doi:10.3390/f14091920

journal article Open Access Sep 21, 2023

Collectible Cell Lines of Larix sibirica Obtained by Somatic Embryogenesis and Their Ability to Regenerate

Iraida N. Tretyakova Maria E. Park

Forests Vol. 14 No. 9 pp. 1920 · MDPI AG

View at Publisher Save 10.3390/f14091920

Abstract

A protocol for the cultivation of Larix sibirica Ledeb. by somatic embryogenesis was developed (RF Patent No. 2456344, 2012). The L. sibirica collection consisted of 22 actively proliferating cell lines (CLs) obtained from immature zygotic embryos. The age of CLs ranged from 1 to 14 years. CLs differed in their growth intensity, embryonic productivity, hormonal balance, and genetic stability, as well as in their regenerative ability. In most proliferating CLs, the formation of globular somatic embryos continued for 2–4 years. Here, a number of CLs actively proliferated for 9–14 years or more. The formation of embryogenic cultures in L. sibirica is associated with the content of phytohormones and their localization in embryo cells. The cytogenetic studies revealed the genetic stability of young CLs (up to 1 year), in which the karyotype consisted of the diploid number of chromosomes (2n = 24). Genomic mutations were observed in the long-term proliferation of CLs. Individual CLs can maintain cytogenetic stability for many years; such CLs can successfully be used to preserve germplasm, obtain planting material, and for plantation reforestation.

Topics

No keywords indexed for this article. Browse by subject →

References

58

[1]

Lelu "An improved method for somatic plantlet production in hybrid larch (Larix × leptoeuropaea): Part 1. Somatic embryo maturation" Plant Cell Tissue Organ Cult. (1994) 10.1007/bf00048321

[2]

Sabala "Developmental pathways of somatic embryogenesis" Plant Cell Tissue Organ Cult. (2002) 10.1023/a:1015673200621

[3]

Park, Y.-S., Bonga, J., McCartney, A., and Adams, G. (2014, January 8–12). Integration of tree biotechnologies into multivarietal forestry. Proceedings of the Third International Conference of the IUFRO Unit 2.09. 02: Somatic Embryogenesis and Other Vegetative Propagation Technologies, Vitoria-Gasteiz, Spain.

[4]

Batygina "Genetic heterogeneity of seeds" Acta Biol. Crac. Ser. Bot. (1999)

[5]

Batygina, T.B. (2014). Biologiya Razvitiya Rastenii (Biology of Plant Development), DEAN. (In Russian).

[6]

Tretyakova "Reproductive potential of conifers, somatic embryogenesis and apomixes" Russ. J. Dev. Biol. (2021) 10.1134/s1062360421020089

[7]

Lelu "An improved method for somatic plantlet production in hybrid larch (Larix × leptoeuropaea): Part 2. Control for germination and plantlet development" Plant Cell Tissue Organ Cult. (1994) 10.1007/bf00048322

[8]

Klimaszewska "Clonal Plant Production from self- and cross-pollinated seed families of Pinus sylvestris (L.) through somatic embryogenesis" Plant Cell Tissue Organ Cult. (2008)

[9]

"Simplified and improved somatic embryogenesis of hybrid larches (Larix × eurolepis and Larix × marschlinsii). Perspectives for breeding" Ann. For. Sci. (2009)

[10]

Klimaszewska "Influence of gelling agents on culture medium gel strength, water availability, tissue water potential, and maturation response in embryogenic cultures of Pinus strobus L." Vitr. Cell. Dev. Biol.-Plant (2000) 10.1007/s11627-000-0051-1

[11]

Cairney "The cellular and molecular biology of conifer embryogenesis" New Phytol. (2007) 10.1111/j.1469-8137.2007.02239.x

[12]

Belorusova "Osobennosti formirovaniya somaticheskih zarodyshej u listvennicy sibirskoj: Embriologicheskie aspekty (Features of the formation of somatic embryos in Siberian larch: Embryological aspects)" Russ. J. Dev. Biol. (2008)

[13]

Pullman "Pine somatic embryogenesis using zygotic embryos as explants" Plant Embryo Culture: Methods and Protocols, Methods in Molecular Biology (2010) 10.1007/978-1-61737-988-8_19

[14]

Barsukova "Somatic embryogenesis in in vitro culture of three larch species" Russ. J. Dev. Biol. (2012) 10.1134/s1062360412060082

[15]

Park "Somatic Polyembriogenesis of Larix sibirica in Embryogenic in vitro Culture" Russ. J. Dev. Biol. (2018) 10.1134/s1062360418040069

[16]

Shuklina "The role of phytohormones in the induction of somatic embryogenesis in Pinus sibirica and Larix sibirica" Cytologia (2021) 10.1508/cytologia.86.55

[17]

Vondrakova "The role of auxins in somatic embryogenesis of Abies alba" Cent. Eur. J. Biol. (2011)

[18]

Vondrakova "Profiles of endogenous phytohormones over the course of Norway spruce somatic embryogenesis" Front. Plant Sci. (2018) 10.3389/fpls.2018.01283

[19]

Jain, S.M., and Gupta, P. (2018). Step Wise Protocols for Somatic Embryogenesis of Important Woody Plants, Springer. [2nd ed.]. 10.1007/978-3-319-89483-6

[20]

Peng, C., Gao, F., Wang, H., Tretyakova, I.N., Nosov, A.M., Shen, H., and Yang, L. (2022). Morphological and physiological indicators for screening cell lines with high potential for somatic embryo maturation at an early stage of somatic embryogenesis in Pinus koraiensis. Plants, 11. 10.3390/plants11141867

[21]

Gao, Y., Cui, Y., Zhao, R., Chen, X., Zhang, J., Zhao, J., and Kong, L. (2022). Cryo-treatment enhances the embryogenicity of mature somatic embryos via the lncRNA–miRNA–mRNA Network in White Spruce. Int. J. Mol. Sci., 23. 10.3390/ijms23031111

[22]

Lambardi "Advances in conifer somatic embryogenesis since year 2000" In Vitro Embryogenesis in Higher Plants, Methods in Molecular Biology (2016)

[23]

Park, Y.S., Beaulieu, J., and Bousquet, J. (2016). Vegetative Propagation of Forest Trees, National Institute of Forest Sciences (NIFoS).

[24]

Bonga, J., Park, Y.S., and Ding, C. (2018, January 10–15). What technical improvements are needed to achieve industrial application of conifer somatic embryogenesis?. Proceedings of the Clonal Trees in the Bioeconomy Age: Opportunities and Challenges, Coimbra, Portugal.

[25]

Ding, C., Park, Y.S., Bonga, J., Bartlett, B., Li, Y., and Raley, F. (2018, January 10–15). A brief review of combining genomic selection and somatic embryogenesis for tree improvement. Proceedings of the Clonal Trees in the Bioeconomy Age: Opportunities and Challenges, Coimbra, Portugal.

[26]

Tretyakova "Osobennosti formirovaniya generativnykh organov listvennitsy sibirskoy i ikh morfogeneticheskiy potentsial (Peculiarities of forming siberian larch generative organs and their morphogenetic potential)" Biol. Bull. Rev. (2006)

[27]

Klimaszewska "Diploid and haploid embryogenesis in Larix leptolepis, L. decidua, and their reciprocal hybrids" Can. J. For. Res. (1990) 10.1139/x90-002

[28]

Ahuja, M.R. (1991). Woody Plant Biotechnology, Springer. 10.1007/978-1-4684-7932-4

[29]

Rupps "Identification of putative homologs of Larix decidua to Baby Boom (BBM), Leafy Cotyledon1 (LEC1), Wuschel-related Homeobox2 (WOX2) and Somatic Embryogenesis Receptor-like Kinase (SERK) during somatic embryogenesis" Planta (2016) 10.1007/s00425-015-2409-y

[30]

Kim "Enhancement of somatic embryogenesis and plant regeneration in Japanese larch (Larix leptolepis)" Plant Cell Tissue Organ Cult. (2007) 10.1007/s11240-007-9202-y

[31]

Li "Regulation of LaMYB33 by miR159 during maintenance of embryogenic potential and somatic embryo maturation in Larix kaempferi (Lamb.) Carr" Plant Cell Tissue Organ Cult. (2013) 10.1007/s11240-012-0233-7

[32]

Thompson "Somatic embryogenesis and plant regeneration from immature embryos of western larch" Plant Cell Rep. (1992) 10.1007/bf00234365

[33]

Zhao "iTRAQ-based comparative proteomic analysis of embryogenic and non-embryogenic tissues of Prince Rupprecht’s larch (Larix principis-rupprechtii Mayr)" Plant Cell Tissue Organ Cult. (2015) 10.1007/s11240-014-0633-y

[34]

Song "Screening and verification of the factors influencing somatic embryo maturation of Larix olgensis" J. For. Res. (2018) 10.1007/s11676-018-0694-6

[35]

Zhang, S., Yan, S., An, P., Cao, Q., Wang, C., Wang, J., Zhang, H., and Zhang, L. (2021). Embryogenic callus induction from immature zygotic embryos and genetic transformation of Larix kaempferi 3× Larix gmelinii 9. PLoS ONE, 16. 10.1371/journal.pone.0258654

[36]

Veselov "Modified solvent partitioning scheme providing increased specificity and rapidity of immunoassay for indole 3-acetic acid" Physiol. Plant (1992) 10.1111/j.1399-3054.1992.tb01316.x

[37]

Kudoyarova "Accumulation of cytokinins in roots and their export to the shoots of durum wheat plants treated with the protonophore carbonyl cyanide m-chlorophenylhydrazone (CCCP)" J. Exp. Bot. (2014) 10.1093/jxb/eru113

[38]

Pak "The embryogenic potential of long-term proliferation cell lines of Larix sibirica in vitro" Sib. Lesn. Zurnal (Sib. J. For. Sci.) (2016)

[39]

Goryachkina "Cytogenetic stability of young and long-term embryogenic cultures of Larix sibirica" Cytologia (2018) 10.1508/cytologia.83.323

[40]

Park "The regenerative capacity of Siberian larch cell lines in vitro" Biol. Bull. Russ. Acad. Sci. (2022) 10.1134/s1062359022050193

[41]

Tretiakova "Embryogenic cell lines and somatic embryogenesis in in vitro culture of Siberian larch" Dokl. Biol. Sci. (2013) 10.1134/s0012496613030034

[42]

Biological characterization of young and aged embryogenic cultures of Pinus pinaster (Ait.)

K. Klimaszewska, C. Noceda, G. Pelletier et al.

In Vitro Cellular & Developmental Biology - Pl... 2009 10.1007/s11627-008-9158-6

[43]

Tretyakova "Peculiarities of somatic embryogenesis of long-term proliferating embryogenic cell lines of Larix sibirica in vitro" Russ. J. Plant Physiol. (2016) 10.1134/s1021443716050137

[44]

Kudoyarova "Content and immunohistochemical localization of hormones during in vitro somatic embryogenesis in long-term proliferating Larix sibirica cultures" Plant Cell Tissue Organ Cult. (2019) 10.1007/s11240-018-01533-y

[45]

Salajova "Somatic embryogenesis in European black pine (Pinus nigra Arn.)" Biol. Plant (1992) 10.1007/bf02925871

[46]

Nkongolo "Cytological and molecular relationships between Larix decidua, L. leptolepis and Larix × eurolepis: Identification of species-specific chromosomes and synchronization of mitotic cell" Theor. Appl. Genet. (1995) 10.1007/bf00222018

[47]

Smith "Flow cytometric determination of genome size in Pinus" Plant Sci. (1996) 10.1016/0168-9452(96)04356-7

[48]

Fourre "Somatic embryogenesis and somaclonal variation in Norway spruce: Morphogenetic, cytogenetic and molecular approaches" Theor. Appl. Genet. (1997) 10.1007/s001220050395

[49]

Park "Somaclonal variation in cryopreserved embryogenic clones of white spruce (Picea glauca (Moench) Voss.)" Plant Cell Rep. (1999) 10.1007/s002990050689

[50]

Marum "Analysis of genetic stability at SSR loci during somatic embryogenesis in maritime pine (Pinus pinaster)" Plant Cell Rep. (2009) 10.1007/s00299-008-0668-9

Showing 50 of 58 references

Metrics

5

Citations

58

References

Details

Published: Sep 21, 2023
Vol/Issue: 14(9)
Pages: 1920
License: View

Authors

I

Iraida N. Tretyakova

Krasnoyarsk Scientific Center of the Siberian Branch of RAS, Division of the Federal Research Center, Sukachev Institute of Forest of the Siberian Branch of RAS, 660036 Krasnoyarsk, Russia

M

Maria E. Park

Krasnoyarsk Scientific Center of the Siberian Branch of RAS, Division of the Federal Research Center, Sukachev Institute of Forest of the Siberian Branch of RAS, 660036 Krasnoyarsk, Russia

Cite This Article

Iraida N. Tretyakova, Maria E. Park (2023). Collectible Cell Lines of Larix sibirica Obtained by Somatic Embryogenesis and Their Ability to Regenerate. Forests, 14(9), 1920. https://doi.org/10.3390/f14091920

Collectible Cell Lines of Larix sibirica Obtained by Somatic Embryogenesis and Their Ability to Regenerate

You May Also Like