Enzymatic Hydrolysis Strategies for Cellulosic Sugars Production to Obtain Bioethanol from Eucalyptus globulus Bark

Mariana S. T. Amândio; Jorge M. S. Rocha; Ana M. R. B. Xavier

doi:10.3390/fermentation9030241

journal article Open Access Mar 02, 2023

Enzymatic Hydrolysis Strategies for Cellulosic Sugars Production to Obtain Bioethanol from Eucalyptus globulus Bark

Mariana S. T. Amândio

Jorge M. S. Rocha

Ana M. R. B. Xavier

Fermentation Vol. 9 No. 3 pp. 241 · MDPI AG

View at Publisher Save 10.3390/fermentation9030241

Abstract

Cellulosic sugars production for the valorization of lignocellulosic biomass residues in an industrial site has economic benefits and is promising if integrated into a biorefinery. Enzymatic hydrolysis (EH) of pretreated Eucalyptus globulus bark, an industrial residue of low-economic value widely available in Portuguese pulp and paper mills, could be an excellent approach to attain resource circularity and pulp mill profitability. This work evaluated the potential for improving cellulosic sugars concentrations by operating with high solids loading and introducing the additives Triton X-100, PEG 4000 and Tween 80 using a commercial enzymatic consortium with a dosage of 25 FPU gcarbohydrates−1. Additives did not improve enzymatic hydrolysis performance, but the effect of increasing solids loading to 14% (w/v) in batch operation was accomplished. The fed-batch operation strategy was investigated and, when starting with 11% (w/v) solids loading, allowed the feeding of 3% (w/v) fresh feedstock sequentially at 2, 4 and 6 h, attaining 20% (w/v) total solids loading. After 24 h of operation, the concentration of cellulosic sugars reached 161 g L−1, corresponding to an EH conversion efficiency of 76%. Finally, the fermentability of the fed-batch hydrolysate using the Ethanol Red® strain was evaluated in a 5 L bioreactor scale. The present results demonstrate that Eucalyptus globulus bark, previously pretreated by kraft pulping, is a promising feedstock for cellulosic sugars production, allowing it to become the raw material for feeding a wide range of bioprocesses.

Topics

No keywords indexed for this article. Browse by subject →

References

82

[1]

Branco, R., Serafim, L., and Xavier, A. (2019). Second Generation Bioethanol Production: On the Use of Pulp and Paper Industry Wastes as Feedstock. Fermentation, 5. 10.3390/fermentation5010004

[2]

Qureshi, N., Hodge, D., and Vertes, A. (2014). Biorefineries, Elsevier.

[3]

Pinto "Improvement of gasification performance of Eucalyptus globulus stumps with torrefaction and densification pre-treatments" Fuel (2017) 10.1016/j.fuel.2017.06.008

[4]

Souza "A New Approach for Conversion of Eucalyptus Lignocellulosic Biomass into Cellulose Nanostructures: A Method that Can Be Applied in Industry" J. Nat. Fibers (2019) 10.1080/15440478.2019.1691125

[5]

Neiva "Potential of Eucalyptus globulus industrial bark as a biorefinery feedstock: Chemical and fuel characterization" Ind. Crop. Prod. (2018) 10.1016/j.indcrop.2018.06.070

[6]

Resquin "Wood quality for kraft pulping of Eucalyptus globulus origins planted in Uruguay" Sci. For./For. Sci. (2006)

[7]

Rodrigues, A.E., Pinto, P.C., Barreiro, M.F., da Costa, C.E., da Mota, M.F., and Fernandes, I. (2018). An Integrated Approach for Added-Value Products from Lignocellulosic Biorefineries: Vanillin, Syringaldehyde, Polyphenols and Polyurethane, Springer. [1st ed.]. 10.1007/978-3-319-99313-3

[8]

Sixta "Wood Yard Operations" Handbook of Pulp (2006)

[9]

Ek, M., Gellerstedt, G., and Henriksson, G. (2009). Pulp and Paper Chemistry and Technology, Walter de Gruyter GmbH & Co. KG. 10.1515/9783110213447

[10]

Biond-Forest Fibers from Portugal (2022, November 28). Boletim Estatístico Biond 2021. Available online: https://www.biond.pt/publicacoes/boletim-estatistico-2021/.

[11]

Domingues "Eucalyptus globulus biomass residues from pulping industry as a source of high value triterpenic compounds" Ind. Crop. Prod. (2010) 10.1016/j.indcrop.2009.09.002

[12]

Neiva "Bark residues valorization potential regarding antioxidant and antimicrobial extracts" Wood Sci. Technol. (2020) 10.1007/s00226-020-01168-3

[13]

Zabed "Bioethanol production from renewable sources: Current perspectives and technological progress" Renew. Sustain. Energy Rev. (2017) 10.1016/j.rser.2016.12.076

[14]

Robak "Review of Second Generation Bioethanol Production from Residual Biomass" Food Technol. Biotechnol. (2018) 10.17113/ftb.56.02.18.5428

[15]

Aditiya "Second generation bioethanol production: A critical review" Renew. Sustain. Energy Rev. (2016) 10.1016/j.rser.2016.07.015

[16]

Fatma "Lignocellulosic Biomass: A Sustainable Bioenergy Source for Future" Protein Pept. Lett. (2018) 10.2174/0929866525666180122144504

[17]

Pino "Bioreactor design for enzymatic hydrolysis of biomass under the biorefinery concept" Chem. Eng. J. (2018) 10.1016/j.cej.2018.04.057

[18]

Improve Enzymatic Hydrolysis of Lignocellulosic Biomass by Modifying Lignin Structure via Sulfite Pretreatment and Using Lignin Blockers

Caoxing Huang, Ruolin Li, Wei Tang et al.

Fermentation 10.3390/fermentation8100558

[19]

Zheng "Pretreatment of lignocellulosic biomass for enhanced biogas production" Prog. Energy. Combust. Sci. (2014) 10.1016/j.pecs.2014.01.001

[20]

Singh, H.B., Gupta, V.K., and Jogaiah, S. (2019). New and Future Developments in Microbial Biotechnology and Bioengineering, Elsevier.

[21]

Padella, M., O’Connell, A., and Prussi, M. (2019). What is still Limiting the Deployment of Cellulosic Ethanol? Analysis of the Current Status of the Sector. Appl. Sci., 9. 10.3390/app9214523

[22]

Qiao "Integrated biorefinery approaches for the industrialization of cellulosic ethanol fuel" Bioresour. Technol. (2022) 10.1016/j.biortech.2022.127516

[23]

Alhammad "Enhancing enzyme-aided production of fermentable sugars from poplar pulp in the presence of non-ionic surfactants" Bioprocess Biosyst. Eng. (2018) 10.1007/s00449-018-1942-z

[24]

"Additives enhancing enzymatic hydrolysis of lignocellulosic biomass" Bioresour. Technol. (2017) 10.1016/j.biortech.2017.06.132

[25]

Peterson "Enhanced enzymatic conversion of softwood lignocellulose by poly(ethylene glycol) addition" Enzyme Microb. Technol. (2007) 10.1016/j.enzmictec.2006.06.006

[26]

Reis "Process strategies to reduce cellulase enzyme loading for renewable sugar production in biorefineries" Chem. Eng. J. (2023) 10.1016/j.cej.2022.138690

[27]

Li "Lignin-enzyme interaction: Mechanism, mitigation approach, modeling, and research prospects" Biotechnol. Adv. (2017) 10.1016/j.biotechadv.2017.03.010

[28]

Florencio "Addition of Soybean Protein Improves Saccharification and Ethanol Production from Hydrothermally Pretreated Sugarcane Bagasse" Bioenergy Res. (2019) 10.1007/s12155-018-9956-6

[29]

Brondi "Alternative Low-Cost Additives to Improve the Saccharification of Lignocellulosic Biomass" Appl. Biochem. Biotechnol. (2019) 10.1007/s12010-018-2834-z

[30]

Mesquita "Alkaline-sulfite pretreatment and use of surfactants during enzymatic hydrolysis to enhance ethanol production from sugarcane bagasse" Bioprocess Biosyst. Eng. (2016) 10.1007/s00449-015-1527-z

[31]

Cheng "Enhanced biodegradation of sugarcane bagasse by Clostridium thermocellum with surfactant addition" Green Chem. (2014) 10.1039/c3gc42494d

[32]

He "Comparison of One-Stage Batch and Fed-Batch Enzymatic Hydrolysis of Pretreated Hardwood for the Production of Biosugar" Appl. Biochem. Biotechnol. (2018) 10.1007/s12010-017-2633-y

[33]

Zhang "Simultaneous saccharification and ethanol fermentation at high corn stover solids loading in a helical stirring bioreactor" Biotechnol. Bioeng. (2010) 10.1002/bit.22593

[34]

Gomes "Fed-batch strategies for saccharification of pilot-scale mild-acid and alkali pretreated sugarcane bagasse: Effects of solid loading and surfactant addition" Ind. Crop. Prod. (2018) 10.1016/j.indcrop.2018.04.026

[35]

Ferreira "Improving the economy of lignocellulose-based biorefineries with organosolv pretreatment" Bioresour. Technol. (2020) 10.1016/j.biortech.2019.122695

[36]

He "Recent advances in membrane technologies for biorefining and bioenergy production" Biotechnol. Adv. (2012) 10.1016/j.biotechadv.2012.01.015

[37]

Guerrero, M. (2017). Portugal Biofuel Market Outlook 2017, USDA Foreign Agricultural Service: Global Agricultural Information Network.

[38]

Hassan "Lignocellulosic Biorefineries in Europe: Current State and Prospects" Trends Biotechnol. (2019) 10.1016/j.tibtech.2018.07.002

[39]

Guigou "Combined pretreatments of eucalyptus sawdust for ethanol production within a biorefinery approach" Biomass Convers. Biorefinery (2019) 10.1007/s13399-018-0353-3

[40]

Sluiter, A., Hames, B., Ruiz, R., Scarlata, C., Sluiter, J., Templeton, D., and Crocker, D. (2021, October 27). Laboratory Analytical Procedure (LAP): Determination of Structural Carbohydrates and Lignin in Biomass, Available online: https://www.nrel.gov/docs/gen/fy13/42618.pdf.

[41]

Adney, B., and Baker, J. (2019, June 30). Laboratory Analytical Procedure (LAP): Measurement of Cellulase Activities, Available online: https://www.nrel.gov/docs/gen/fy08/42628.pdf.

[42]

Branco, R.H.R., Amândio, M.S.T., Serafim, L.S., and Xavier, A.M.R.B. (2020). Ethanol production from hydrolyzed kraft pulp by mono- and co-cultures of yeasts: The challenge of C6 and C5 sugars consumption. Energies, 13. 10.3390/en13030744

[43]

Raj "Improved high solid loading enzymatic hydrolysis of low-temperature aqueous ammonia soaked sugarcane bagasse using laccase-mediator system and high concentration ethanol production" Ind. Crop. Prod. (2019) 10.1016/j.indcrop.2019.01.032

[44]

Gao "Optimization of fed-batch enzymatic hydrolysis from alkali-pretreated sugarcane bagasse for high-concentration sugar production" Bioresour. Technol. (2014) 10.1016/j.biortech.2014.05.034

[45]

Kang "Bioethanol from lignocellulosic biomass: Current findings determine research priorities" Sci. World J. (2014) 10.1155/2014/298153

[46]

Oliveira "The impact of acid hydrolysis conditions on carbohydrate determination in lignocellulosic materials: A case study with Eucalyptus globulus bark" Holzforschung (2021) 10.1515/hf-2020-0250

[47]

Pinto "Performance of Side-Streams from Eucalyptus Processing as Sources of Polysaccharides and Lignins by Kraft Delignification" Ind. Eng. Chem. Res. (2016) 10.1021/acs.iecr.5b03712

[48]

Monrroy "Kraft pulping of Eucalyptus globulus as a pretreatment for bioethanol production by simultaneous saccharification and fermentation" J. Chil. Chem. Soc. (2012) 10.4067/s0717-97072012000200012

[49]

"Pretreatment of lignocellulose: Formation of inhibitory by-products and strategies for minimizing their effects" Bioresour. Technol. (2016) 10.1016/j.biortech.2015.10.009

[50]

Ko "Potentials of lignocellulosic bioethanols produced from hardwood in Taiwan" Energy (2012) 10.1016/j.energy.2012.06.026

Showing 50 of 82 references

Cited By

45

Production and characterization of sophorolipid under yeast-mediated submerged fermentation utilizing Agro-industrial waste

Aishwary Purohit, Amar Jyoti Das · 2025

Current Research in Microbial Scien...

Yarrowia lipolytica uses olive tree prunings hydrolysates for microbial lipids as biodiesel feedstock

Bruna Dias, Rosa M. Rodríguez-Jasso · 2025

Chemical Engineering Science

Effect of enzyme hydrolysis on the physicochemical, functional, and nutritional properties of pea and faba bean protein isolates

Joy Sareen, Dai Shi · 2023

European Food Research and Technolo...

Metrics

45

Citations

82

References

Details

Published: Mar 02, 2023
Vol/Issue: 9(3)
Pages: 241
License: View

Authors

M

Mariana S. T. Amândio

CICECO—Aveiro Institute of Materials, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal; CIEPQPF, Department of Chemical Engineering, Faculty of Sciences and Technology, University of Coimbra, 3030-790 Coimbra, Portugal

J

Jorge M. S. Rocha

CIEPQPF, Department of Chemical Engineering, Faculty of Sciences and Technology, University of Coimbra, 3030-790 Coimbra, Portugal

A

Ana M. R. B. Xavier

CICECO—Aveiro Institute of Materials, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal

Funding

National Funds Award: 21874

COMPETE 2020 Award: 21874

project CICECO-Aveiro Institute of Materials Award: 21874

InPaCTus—Innovative Products and Technologies from Eucalyptus Award: 21874

Portugal 2020 through European Regional Development Fund (ERDF) Award: 21874

CIEPQPF—Strategic Research Centre Project Award: 21874

Cite This Article

Mariana S. T. Amândio, Jorge M. S. Rocha, Ana M. R. B. Xavier (2023). Enzymatic Hydrolysis Strategies for Cellulosic Sugars Production to Obtain Bioethanol from Eucalyptus globulus Bark. Fermentation, 9(3), 241. https://doi.org/10.3390/fermentation9030241

Enzymatic Hydrolysis Strategies for Cellulosic Sugars Production to Obtain Bioethanol from Eucalyptus globulus Bark

You May Also Like