Production and New Extraction Method of Polyketide Red Pigments Produced by Ascomycetous Fungi from Terrestrial and Marine Habitats

Juliana Lebeau; Mekala Venkatachalam; Mireille Fouillaud; Thomas Petit; Francesco Vinale; Laurent Dufossé; Yanis Caro

doi:10.3390/jof3030034

journal article Open Access Jun 28, 2017

Production and New Extraction Method of Polyketide Red Pigments Produced by Ascomycetous Fungi from Terrestrial and Marine Habitats

Juliana Lebeau Mekala Venkatachalam

Mireille Fouillaud

Thomas Petit Francesco Vinale

Laurent Dufossé

Yanis Caro

Journal of Fungi Vol. 3 No. 3 pp. 34 · MDPI AG

View at Publisher Save 10.3390/jof3030034

Abstract

The use of ascomycetous fungi as pigment producers opens the way to an alternative to synthetic dyes, especially in the red-dye industries, which have very few natural pigment alternatives. The present paper aimed to bio-prospect and screen out 15 selected ascomycetous fungal strains, originating from terrestrial and marine habitats belonging to seven different genera (Penicillium, Talaromyces, Fusarium, Aspergillus, Trichoderma, Dreschlera, and Paecilomyces). We identified four strains, Penicillium purpurogenum rubisclerotium, Fusarium oxysporum, marine strains identified as Talaromyces spp., and Trichoderma atroviride, as potential red pigment producers. The extraction of the pigments is a crucial step, whereby the qualitative and quantitative compositions of each fungal extract need to be respected for reliable identification, as well as preserving bioactivity. Furthermore, there is a growing demand for more sustainable and cost-effective extraction methods. Therefore, a pressurized liquid extraction technique was carried out in this study, allowing a greener and faster extraction step of the pigments, while preserving their chemical structures and bioactivities in comparison to conventional extraction processes. The protocol was illustrated with the production of pigment extracts from P. purpurogenum rubisclerotium and Talaromyces spp. Extracts were analyzed by high-performance liquid-chromatography combined with photodiode array-detection (HPLC-DAD) and high-resolution mass spectrometry (UHPLC-HRMS). The more promising strain was the isolate Talaromyces spp. of marine origin. The main polyketide pigment produced by this strain has been characterized as N-threoninerubropunctamine, a non-toxic red Monascus-like azaphilone pigment.

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References

Details

Published: Jun 28, 2017
Vol/Issue: 3(3)
Pages: 34
License: View

Authors

J

Juliana Lebeau

Laboratoire de Chimie des Substances Naturelles et des Sciences des Aliments (LCSNSA), Université de la Réunion, F-97490 Sainte-Clotilde, Ile de la Réunion, France

M

Mekala Venkatachalam

Laboratoire de Chimie des Substances Naturelles et des Sciences des Aliments (LCSNSA), Université de la Réunion, F-97490 Sainte-Clotilde, Ile de la Réunion, France

M

Mireille Fouillaud

Laboratoire de Chimie des Substances Naturelles et des Sciences des Aliments (LCSNSA), Université de la Réunion, F-97490 Sainte-Clotilde, Ile de la Réunion, France

T

Thomas Petit

UMR QualiSud, Université de la Réunion, IUT, F-97410 Saint-Pierre, Ile de la Réunion, France

F

Francesco Vinale

Istituto per la Protezione Sostenibile delle Piante (IPSP-CNR) and Dipartimento di Agraria, Università degli Studi di Napoli Federico II, 80055 Portici, NA, Italy

L

Laurent Dufossé

Laboratoire de Chimie des Substances Naturelles et des Sciences des Aliments (LCSNSA), Université de la Réunion, F-97490 Sainte-Clotilde, Ile de la Réunion, France

Y

Yanis Caro

Laboratoire de Chimie des Substances Naturelles et des Sciences des Aliments (LCSNSA), Université de la Réunion, F-97490 Sainte-Clotilde, Ile de la Réunion, France

Cite This Article

Juliana Lebeau, Mekala Venkatachalam, Mireille Fouillaud, et al. (2017). Production and New Extraction Method of Polyketide Red Pigments Produced by Ascomycetous Fungi from Terrestrial and Marine Habitats. Journal of Fungi, 3(3), 34. https://doi.org/10.3390/jof3030034

Production and New Extraction Method of Polyketide Red Pigments Produced by Ascomycetous Fungi from Terrestrial and Marine Habitats

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