Bio-Synthesis of Aspergillus terreus Mediated Gold Nanoparticle: Antimicrobial, Antioxidant, Antifungal and In Vitro Cytotoxicity Studies

Rahul Chandra Mishra; Rishu Kalra; Rahul Dilawari; Mayurika Goel; Colin J. Barrow

doi:10.3390/ma15113877

journal article Open Access May 29, 2022

Bio-Synthesis of Aspergillus terreus Mediated Gold Nanoparticle: Antimicrobial, Antioxidant, Antifungal and In Vitro Cytotoxicity Studies

Rahul Chandra Mishra Rishu Kalra Rahul Dilawari

Mayurika Goel Colin J. Barrow

Materials Vol. 15 No. 11 pp. 3877 · MDPI AG

View at Publisher Save 10.3390/ma15113877

Abstract

Gold nanoparticles (GNP) were bio-fabricated utilizing the methanolic extract of the endophytic isolate Aspergillus terreus. The biosynthesised gold nanoparticles (GNP023) were characterised using UV-visible spectroscopy (UV-Vis); transmission electron microscopy (TEM), Fourier-transform nfrared spectroscopy (FTIR) and X-ray diffraction (XRD) studies. The bio-fabricated GNP023 displayed a sharp SPR peak at 536 nm, were spherically shaped, and had an average size between 10–16 nm. The EDX profile confirmed the presence of gold (Au), and XRD analysis confirmed the crystalline nature of GNP023. The antimicrobial activity of GNP023 was investigated against several food-borne and phytopathogens, using in vitro antibacterial and antifungal assays. The maximum zone of inhibition was observed for S. aureus and V. cholera at 400 μg /mL, whereas inhibition in radial mycelial growth was observed against Fusarium oxysporum and Rhizoctonia solani at 52.5% and 65.46%, respectively, when challenged with GNP023 (200 μg/mL). Moreover, the gold nanoparticles displayed significant antioxidant activity against the ABTS radical, with an IC50 of 38.61 µg/mL, and were non-toxic when tested against human kidney embryonic 293 (HEK293) cells. Thus, the current work supports the application of myco-synthesised gold nanoparticles as a versatile antimicrobial candidate against food-borne pathogens.

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Details

Published: May 29, 2022
Vol/Issue: 15(11)
Pages: 3877
License: View

Authors

R

Rahul Chandra Mishra

TERI-Deakin Nano Biotechnology Centre, The Energy and Resources Institute (TERI), TERI GRAM, Gurgaon 122001, India; Centre for Bioprocessing, School of Life and Environmental Sciences, Deakin University, Waurn Ponds, VIC 3220, Australia

R

Rishu Kalra

TERI-Deakin Nano Biotechnology Centre, The Energy and Resources Institute (TERI), TERI GRAM, Gurgaon 122001, India

R

Rahul Dilawari

CSIR-Institute of Microbial Technology, Sector-39-A, Chandigarh 160036, India

M

Mayurika Goel

TERI-Deakin Nano Biotechnology Centre, The Energy and Resources Institute (TERI), TERI GRAM, Gurgaon 122001, India

C

Colin J. Barrow

Centre for Bioprocessing, School of Life and Environmental Sciences, Deakin University, Waurn Ponds, VIC 3220, Australia

Funding

Deakin University

Energy and Resources Institute

Deakin University HDR scholarship

Cite This Article

Rahul Chandra Mishra, Rishu Kalra, Rahul Dilawari, et al. (2022). Bio-Synthesis of Aspergillus terreus Mediated Gold Nanoparticle: Antimicrobial, Antioxidant, Antifungal and In Vitro Cytotoxicity Studies. Materials, 15(11), 3877. https://doi.org/10.3390/ma15113877

Bio-Synthesis of Aspergillus terreus Mediated Gold Nanoparticle: Antimicrobial, Antioxidant, Antifungal and In Vitro Cytotoxicity Studies

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