Genomic Analysis of Novel Sulfitobacter Bacterial Strains Isolated from Marine Biofilms

Han Cui; Shen Fan; Wei Ding; Weipeng Zhang

doi:10.3390/md22070289

journal article Open Access Jun 22, 2024

Genomic Analysis of Novel Sulfitobacter Bacterial Strains Isolated from Marine Biofilms

Han Cui

Shen Fan Wei Ding

Weipeng Zhang

Marine Drugs Vol. 22 No. 7 pp. 289 · MDPI AG

View at Publisher Save 10.3390/md22070289

Abstract

Bacteria from the genus Sulfitobacter are distributed across various marine habitats and play a significant role in sulfur cycling. However, the metabolic features of Sulfitobacter inhabiting marine biofilms are still not well understood. Here, complete genomes and paired metatranscriptomes of eight Sulfitobacter strains, isolated from biofilms on subtidal stones, have been analyzed to explore their central energy metabolism and potential of secondary metabolite biosynthesis. Based on average nucleotide identity and phylogenetic analysis, the eight strains were classified into six novel species and two novel strains. The reconstruction of the metabolic pathways indicated that all strains had a complete Entner–Doudoroff pathway, pentose phosphate pathway, and diverse pathways for amino acid metabolism, suggesting the presence of an optimized central carbon metabolism. Pangenome analysis further revealed the differences between the gene cluster distribution patterns among the eight strains, suggesting significant functional variation. Moreover, a total of 47 biosynthetic gene clusters were discovered, which were further classified into 37 gene cluster families that showed low similarity with previously documented clusters. Furthermore, metatranscriptomic analysis revealed the expressions of key functional genes involved in the biosynthesis of ribosomal peptides in in situ marine biofilms. Overall, this study sheds new light on the metabolic features, adaptive strategies, and value of genome mining in this group of biofilm-associated Sulfitobacter bacteria.

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Details

Published: Jun 22, 2024
Vol/Issue: 22(7)
Pages: 289
License: View

Authors

H

Han Cui

MOE Key Laboratory of Marine Genetics & Breeding and College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China; MOE Key Laboratory of Evolution & Marine Biodiversity and Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao 266003, China

S

Shen Fan

MOE Key Laboratory of Marine Genetics & Breeding and College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China; MOE Key Laboratory of Evolution & Marine Biodiversity and Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao 266003, China

W

Wei Ding

MOE Key Laboratory of Evolution & Marine Biodiversity and Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao 266003, China

W

Weipeng Zhang

MOE Key Laboratory of Marine Genetics & Breeding and College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China

Funding

Fundamental Research Funds for the Central Universities Award: 4022000-842441005

Research Funds from Shandong Province Award: 4022000-842441005

Cite This Article

Han Cui, Shen Fan, Wei Ding, et al. (2024). Genomic Analysis of Novel Sulfitobacter Bacterial Strains Isolated from Marine Biofilms. Marine Drugs, 22(7), 289. https://doi.org/10.3390/md22070289

Genomic Analysis of Novel Sulfitobacter Bacterial Strains Isolated from Marine Biofilms

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