Synthetic Biology Toolkit for a New Species of Pseudomonas Promissory for Electricity Generation in Microbial Fuel Cells

Franciene Rabiço; Matheus Pedrino; Julia Pereira Narcizo; Adalgisa Rodrigues de Andrade; Valeria Reginatto; María-Eugenia Guazzaroni

doi:10.3390/microorganisms11082044

journal article Open Access Aug 09, 2023

Synthetic Biology Toolkit for a New Species of Pseudomonas Promissory for Electricity Generation in Microbial Fuel Cells

Franciene Rabiço Matheus Pedrino Julia Pereira Narcizo

Adalgisa Rodrigues de Andrade

Valeria Reginatto

María-Eugenia Guazzaroni

Microorganisms Vol. 11 No. 8 pp. 2044 · MDPI AG

View at Publisher Save 10.3390/microorganisms11082044

Abstract

Microbial fuel cells (MFCs) offer sustainable solutions for various biotechnological applications and are a crucial area of research in biotechnology. MFCs can effectively treat various refuse, such as wastewater and biodiesel waste by decomposing organic matter and generating electricity. Certain Pseudomonas species possess extracellular electron transfer (EET) pathways, enabling them to transfer electrons from organic compounds to the MFC’s anode. Moreover, Pseudomonas species can grow under low-oxygen conditions, which is advantageous considering that the electron transfer process in an MFC typically leads to reduced oxygen levels at the anode. This study focuses on evaluating MFCs inoculated with a new Pseudomonas species grown with 1 g.L−1 glycerol, a common byproduct of biodiesel production. Pseudomonas sp. BJa5 exhibited a maximum power density of 39 mW.m−2. Also, the observed voltammograms and genome analysis indicate the potential production of novel redox mediators by BJa5. Additionally, we investigated the bacterium’s potential as a synthetic biology non-model chassis. Through testing various genetic parts, including constitutive promoters, replication origins and cargos using pSEVA vectors as a scaffold, we assessed the bacterium’s suitability. Overall, our findings offer valuable insights into utilizing Pseudomonas spp. BJa5 as a novel chassis for MFCs. Synthetic biology approaches can further enhance the performance of this bacterium in MFCs, providing avenues for improvement.

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Details

Published: Aug 09, 2023
Vol/Issue: 11(8)
Pages: 2044
License: View

Authors

F

Franciene Rabiço

Department of Biology, Faculty of Philosophy, Sciences and Letters of Ribeirão Preto, University of São Paulo, Ribeirão Preto 14040-901, Brazil

M

Matheus Pedrino

Department of Biology, Faculty of Philosophy, Sciences and Letters of Ribeirão Preto, University of São Paulo, Ribeirão Preto 14040-901, Brazil

J

Julia Pereira Narcizo

Department of Chemistry, Faculty of Philosophy, Sciences and Letters of Ribeirão Preto, University of São Paulo, Ribeirão Preto 14049-900, Brazil

A

Adalgisa Rodrigues de Andrade

Department of Chemistry, Faculty of Philosophy, Sciences and Letters of Ribeirão Preto, University of São Paulo, Ribeirão Preto 14049-900, Brazil

V

Valeria Reginatto

Department of Chemistry, Faculty of Philosophy, Sciences and Letters of Ribeirão Preto, University of São Paulo, Ribeirão Preto 14049-900, Brazil

M

María-Eugenia Guazzaroni

Department of Biology, Faculty of Philosophy, Sciences and Letters of Ribeirão Preto, University of São Paulo, Ribeirão Preto 14040-901, Brazil

Funding

National Council for Scientific and Technological Development Award: 2014/50924-4

CNPq scholarship Award: 2014/50924-4

Cite This Article

Franciene Rabiço, Matheus Pedrino, Julia Pereira Narcizo, et al. (2023). Synthetic Biology Toolkit for a New Species of Pseudomonas Promissory for Electricity Generation in Microbial Fuel Cells. Microorganisms, 11(8), 2044. https://doi.org/10.3390/microorganisms11082044

Synthetic Biology Toolkit for a New Species of Pseudomonas Promissory for Electricity Generation in Microbial Fuel Cells

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