Fecal Microbial Composition and Predicted Functional Profile in Irritable Bowel Syndrome Differ between Subtypes and Geographical Locations

Jose F. Garcia-Mazcorro; Mercedes Amieva-Balmori; Arturo Triana-Romero; Bridgette Wilson; Leanne Smith; Job Reyes-Huerta; Megan Rossi; Kevin Whelan; Jose M. Remes-Troche

doi:10.3390/microorganisms11102493

journal article Open Access Oct 05, 2023

Fecal Microbial Composition and Predicted Functional Profile in Irritable Bowel Syndrome Differ between Subtypes and Geographical Locations

Jose F. Garcia-Mazcorro

Mercedes Amieva-Balmori Arturo Triana-Romero

Bridgette Wilson Leanne Smith Job Reyes-Huerta Megan Rossi Kevin Whelan Jose M. Remes-Troche

Microorganisms Vol. 11 No. 10 pp. 2493 · MDPI AG

View at Publisher Save 10.3390/microorganisms11102493

Abstract

Increasing evidence suggests a microbial pathogenesis in irritable bowel syndrome (IBS) but the relationship remains elusive. Fecal DNA samples from 120 patients with IBS, 82 Mexican (IBS-C: n = 33, IBS-D: n = 24, IBS-M: n = 25) and 38 British (IBS-C: n = 6, IBS-D: n = 27, IBS-M: n = 5), were available for analysis using 16S rRNA gene sequencing. Firmicutes (mean: 82.1%), Actinobacteria (10.2%), and Bacteroidetes (4.4%) were the most abundant taxa. The analysis of all samples (n = 120), and females (n = 94) only, showed no significant differences in bacterial microbiota, but the analysis of Mexican patients (n = 82) showed several differences in key taxa (e.g., Faecalibacterium) among the different IBS subtypes. In IBS-D there were significantly higher Bacteroidetes in British patients (n = 27) than in Mexican patients (n = 24), suggesting unique fecal microbiota signatures within the same IBS subtype. These differences in IBS-D were also observed at lower phylogenetic levels (e.g., higher Enterobacteriaceae and Streptococcus in Mexican patients) and were accompanied by differences in several alpha diversity metrics. Beta diversity was not different among IBS subtypes when using all samples, but the analysis of IBS-D patients revealed consistent differences between Mexican and British patients. This study suggests that fecal microbiota is different between IBS subtypes and also within each subtype depending on geographical location.

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Details

Published: Oct 05, 2023
Vol/Issue: 11(10)
Pages: 2493
License: View

Authors

J

Jose F. Garcia-Mazcorro

Research and Development, MNA de Mexico, San Nicolas de los Garza 66477, Mexico

M

Mercedes Amieva-Balmori

Instituto de Investigaciones Médico Biológicas, Universidad Veracruzana, Veracruz 91700, Mexico

A

Arturo Triana-Romero

Instituto de Investigaciones Médico Biológicas, Universidad Veracruzana, Veracruz 91700, Mexico

B

Bridgette Wilson

Department of Nutritional Sciences, King’s College London, London WC2R 2LS, UK

L

Leanne Smith

Department of Nutritional Sciences, King’s College London, London WC2R 2LS, UK

J

Job Reyes-Huerta

Instituto de Investigaciones Médico Biológicas, Universidad Veracruzana, Veracruz 91700, Mexico

M

Megan Rossi

Department of Nutritional Sciences, King’s College London, London WC2R 2LS, UK

K

Kevin Whelan

Department of Nutritional Sciences, King’s College London, London WC2R 2LS, UK

J

Jose M. Remes-Troche

Instituto de Investigaciones Médico Biológicas, Universidad Veracruzana, Veracruz 91700, Mexico

Funding

CONACYT Award: MR/N029097/1

Newton Fund Award: MR/N029097/1

Cite This Article

Jose F. Garcia-Mazcorro, Mercedes Amieva-Balmori, Arturo Triana-Romero, et al. (2023). Fecal Microbial Composition and Predicted Functional Profile in Irritable Bowel Syndrome Differ between Subtypes and Geographical Locations. Microorganisms, 11(10), 2493. https://doi.org/10.3390/microorganisms11102493

Fecal Microbial Composition and Predicted Functional Profile in Irritable Bowel Syndrome Differ between Subtypes and Geographical Locations

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