The Endophytic Fungus Chaetomium cupreum Regulates Expression of Genes Involved in the Tolerance to Metals and Plant Growth Promotion in Eucalyptus globulus Roots

Javier Ortiz; Javiera Soto; Alejandra Fuentes; Héctor Herrera; Claudio Meneses; César Arriagada

doi:10.3390/microorganisms7110490

journal article Open Access Oct 26, 2019

The Endophytic Fungus Chaetomium cupreum Regulates Expression of Genes Involved in the Tolerance to Metals and Plant Growth Promotion in Eucalyptus globulus Roots

Javier Ortiz Javiera Soto Alejandra Fuentes Héctor Herrera

Claudio Meneses

César Arriagada

Microorganisms Vol. 7 No. 11 pp. 490 · MDPI AG

View at Publisher Save 10.3390/microorganisms7110490

Abstract

The endophytic strain Chaetomium cupreum isolated from metal-contaminated soil was inoculated in Eucalyptus globulus roots to identify genes involved in metal stress response and plant growth promotion. We analyzed the transcriptome of E. globulus roots inoculated with C. cupreum. De novo sequencing, assembly, and analysis were performed to identify molecular mechanisms involved in metal stress tolerance and plant growth promotion. A total of 393,371,743 paired-end reads were assembled into 135,155 putative transcripts. It was found that 663 genes significantly changed their expression in the presence of treatment, of which 369 were up-regulated and 294 were down-regulated. We found differentially expressed genes (DEGs) encoding metal transporters, transcription factors, stress and defense response proteins, as well as DEGs involved in auxin biosynthesis and metabolism. Our results showed that the inoculation of C. cupreum enhanced tolerance to metals and growth promotion on E. globulus. This study provides new information to understand molecular mechanisms involved in plant–microbe interactions under metals stress.

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References

Details

Published: Oct 26, 2019
Vol/Issue: 7(11)
Pages: 490
License: View

Authors

J

Javier Ortiz

Laboratorio de Biorremediación, Facultad de Ciencias Agropecuarias y Forestales, Universidad de La Frontera, Francisco Salazar 01145, Temuco, Chile; Programa de Doctorado en Ciencias Mención Biología Celular y Molecular Aplicada, Universidad de La Frontera, Francisco Salazar 01145, Temuco, Chile

J

Javiera Soto

Laboratorio de Biorremediación, Facultad de Ciencias Agropecuarias y Forestales, Universidad de La Frontera, Francisco Salazar 01145, Temuco, Chile; Programa de Doctorado en Ciencias Mención Biología Celular y Molecular Aplicada, Universidad de La Frontera, Francisco Salazar 01145, Temuco, Chile

A

Alejandra Fuentes

Laboratorio de Biorremediación, Facultad de Ciencias Agropecuarias y Forestales, Universidad de La Frontera, Francisco Salazar 01145, Temuco, Chile

H

Héctor Herrera

Laboratorio de Biorremediación, Facultad de Ciencias Agropecuarias y Forestales, Universidad de La Frontera, Francisco Salazar 01145, Temuco, Chile

C

Claudio Meneses

Centro de Biotecnología Vegetal, Facultad de Ciencias Biológicas, Universidad Andrés Bello, Santiago, Chile

C

César Arriagada

Laboratorio de Biorremediación, Facultad de Ciencias Agropecuarias y Forestales, Universidad de La Frontera, Francisco Salazar 01145, Temuco, Chile

Funding

Fondo Nacional de Desarrollo Cientìfico y Tecnológico Award: 1170931

Cite This Article

Javier Ortiz, Javiera Soto, Alejandra Fuentes, et al. (2019). The Endophytic Fungus Chaetomium cupreum Regulates Expression of Genes Involved in the Tolerance to Metals and Plant Growth Promotion in Eucalyptus globulus Roots. Microorganisms, 7(11), 490. https://doi.org/10.3390/microorganisms7110490

The Endophytic Fungus Chaetomium cupreum Regulates Expression of Genes Involved in the Tolerance to Metals and Plant Growth Promotion in Eucalyptus globulus Roots

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