High Colonization Rate and Heterogeneity of ESBL- and Carbapenemase-Producing Enterobacteriaceae Isolated from Gull Feces in Lisbon, Portugal

Marta Aires-de-Sousa; Claudine Fournier; Elizeth Lopes; Hermínia de Lencastre; Patrice Nordmann; Laurent Poirel

doi:10.3390/microorganisms8101487

journal article Open Access Sep 28, 2020

High Colonization Rate and Heterogeneity of ESBL- and Carbapenemase-Producing Enterobacteriaceae Isolated from Gull Feces in Lisbon, Portugal

Marta Aires-de-Sousa

Claudine Fournier

Elizeth Lopes

Hermínia de Lencastre Patrice Nordmann

Laurent Poirel

Microorganisms Vol. 8 No. 10 pp. 1487 · MDPI AG

View at Publisher Save 10.3390/microorganisms8101487

Abstract

In order to evaluate whether seagulls living on the Lisbon coastline, Portugal, might be colonized and consequently represent potential spreaders of multidrug-resistant bacteria, a total of 88 gull fecal samples were screened for detection of extended-spectrum β-lactamase (ESBL)- or carbapenemase-producing Enterobacteriaceae for methicillin-resistant Staphylococcus aureus (MRSA) and for vancomycin-resistant Enterococci (VRE). A large proportion of samples yielded carbapenemase- or ESBL-producing Enterobacteriaceae (16% and 55%, respectively), while only two MRSA and two VRE were detected. Mating-out assays followed by PCR and whole-plasmid sequencing allowed to identify carbapenemase and ESBL encoding genes. Among 24 carbapenemase-producing isolates, there were mainly Klebsiella pneumoniae (50%) and Escherichia coli (33%). OXA-181 was the most common carbapenemase identified (54%), followed by OXA-48 (25%) and KPC-2 (17%). Ten different ESBLs were found among 62 ESBL-producing isolates, mainly being CTX-M-type enzymes (87%). Co-occurrence in single samples of multiple ESBL- and carbapenemase producers belonging to different bacterial species was observed in some cases. Seagulls constitute an important source for spreading multidrug-resistant bacteria in the environment and their gut microbiota a formidable microenvironment for transfer of resistance genes within bacterial species.

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References

Details

Published: Sep 28, 2020
Vol/Issue: 8(10)
Pages: 1487
License: View

Authors

M

Marta Aires-de-Sousa

Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, CH-1700 Fribourg, Switzerland; Escola Superior de Saúde da Cruz Vermelha Portuguesa, 1300 Lisbon, Portugal; Laboratory of Molecular Genetics, Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, 2780 Oeiras, Portugal

C

Claudine Fournier

Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, CH-1700 Fribourg, Switzerland

E

Elizeth Lopes

Laboratory of Molecular Genetics, Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, 2780 Oeiras, Portugal

H

Hermínia de Lencastre

Laboratory of Molecular Genetics, Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, 2780 Oeiras, Portugal; Laboratory of Microbiology and Infectious Diseases, The Rockefeller University, New York, NY 10065, USA

P

Patrice Nordmann

Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, CH-1700 Fribourg, Switzerland; Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), CH-1700 Fribourg, Switzerland

L

Laurent Poirel

Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, CH-1700 Fribourg, Switzerland; Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), CH-1700 Fribourg, Switzerland

Funding

Faculté des Sciences et de Médecine, Université de Fribourg Award: 00

Cite This Article

Marta Aires-de-Sousa, Claudine Fournier, Elizeth Lopes, et al. (2020). High Colonization Rate and Heterogeneity of ESBL- and Carbapenemase-Producing Enterobacteriaceae Isolated from Gull Feces in Lisbon, Portugal. Microorganisms, 8(10), 1487. https://doi.org/10.3390/microorganisms8101487

High Colonization Rate and Heterogeneity of ESBL- and Carbapenemase-Producing Enterobacteriaceae Isolated from Gull Feces in Lisbon, Portugal

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