Unraveling the Central Role of Sulfur-Oxidizing Acidiphilium multivorum LMS in Industrial Bioprocessing of Gold-Bearing Sulfide Concentrates

Anna Panyushkina; Aleksandr Bulaev; Aleksandr V. Belyi

doi:10.3390/microorganisms9050984

journal article Open Access May 01, 2021

Unraveling the Central Role of Sulfur-Oxidizing Acidiphilium multivorum LMS in Industrial Bioprocessing of Gold-Bearing Sulfide Concentrates

Anna Panyushkina

Aleksandr Bulaev

Aleksandr V. Belyi

Microorganisms Vol. 9 No. 5 pp. 984 · MDPI AG

View at Publisher Save 10.3390/microorganisms9050984

Abstract

Acidiphilium multivorum LMS is an acidophile isolated from industrial bioreactors during the processing of the gold-bearing pyrite-arsenopyrite concentrate at 38–42 °C. Most strains of this species are obligate organoheterotrophs that do not use ferrous iron or reduced sulfur compounds as energy sources. However, the LMS strain was identified as one of the predominant sulfur oxidizers in acidophilic microbial consortia. In addition to efficient growth under strictly heterotrophic conditions, the LMS strain proved to be an active sulfur oxidizer both in the presence or absence of organic compounds. Interestingly, Ac. multivorum LMS was able to succeed more common sulfur oxidizers in microbial populations, which indicated a previously underestimated role of this bacterium in industrial bioleaching operations. In this study, the first draft genome of the sulfur-oxidizing Ac. multivorum was sequenced and annotated. Based on the functional genome characterization, sulfur metabolism pathways were reconstructed. The LMS strain possessed a complicated multi-enzyme system to oxidize elemental sulfur, thiosulfate, sulfide, and sulfite to sulfate as the final product. Altogether, the phenotypic description and genome analysis unraveled a crucial role of Ac. multivorum in some biomining processes and revealed unique strain-specific characteristics, including the ars genes conferring arsenic resistance, which are similar to those of phylogenetically distinct microorganisms.

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Details

Published: May 01, 2021
Vol/Issue: 9(5)
Pages: 984
License: View

Authors

A

Anna Panyushkina

Winogradsky Institute of Microbiology, Research Centre of Biotechnology of the Russian Academy of Sciences, Leninsky Ave., 33, bld. 2, 119071 Moscow, Russia

A

Aleksandr Bulaev

Winogradsky Institute of Microbiology, Research Centre of Biotechnology of the Russian Academy of Sciences, Leninsky Ave., 33, bld. 2, 119071 Moscow, Russia

A

Aleksandr V. Belyi

JSC “Polyus Krasnoyarsk”, Research Center, Poligonnaya Str., 15, 660118 Krasnoyarsk, Russia

Funding

Ministry of Science and Higher Education of the Russian Federation Award: .

Cite This Article

Anna Panyushkina, Aleksandr Bulaev, Aleksandr V. Belyi (2021). Unraveling the Central Role of Sulfur-Oxidizing Acidiphilium multivorum LMS in Industrial Bioprocessing of Gold-Bearing Sulfide Concentrates. Microorganisms, 9(5), 984. https://doi.org/10.3390/microorganisms9050984

Unraveling the Central Role of Sulfur-Oxidizing Acidiphilium multivorum LMS in Industrial Bioprocessing of Gold-Bearing Sulfide Concentrates

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