Cinnamaldehyde Resist Salmonella Typhimurium Adhesion by Inhibiting Type I Fimbriae

Lizi Yin; Yuyun Dai; Han Chen; Xuewen He; Ping Ouyang; Xiaoli Huang; Xiangang Sun; Yanru Ai; Siyuan Lai; Ling Zhu; Zhiwen Xu

doi:10.3390/molecules27227753

journal article Open Access Nov 10, 2022

Cinnamaldehyde Resist Salmonella Typhimurium Adhesion by Inhibiting Type I Fimbriae

Lizi Yin

Yuyun Dai Han Chen

Xuewen He

Ping Ouyang Xiaoli Huang

Xiangang Sun Yanru Ai Siyuan Lai Ling Zhu

Zhiwen Xu

Molecules Vol. 27 No. 22 pp. 7753 · MDPI AG

View at Publisher Save 10.3390/molecules27227753

Abstract

Salmonella Typhimurium (S. Typhimurium), a common foodborne pathogen, severely harms the public and food security. Type I fimbriae (T1F) of S. Typhimurium, plays a crucial role in the pathogenic processes; it mediates the adhesion of bacteria to the mannose receptor on the host cell, assists the bacteria to invade the host cell, and triggers an inflammatory response. Cinnamaldehyde is the main ingredient in cinnamon essential oil. In this study, cinnamaldehyde was demonstrated to inhibit the expression of T1F by hemagglutination inhibition test, transmission electron microscopy, and biofilms. The mechanism of cinnamaldehyde action was studied by proteomics technology, PCR and Western blotting. The results showed that cinnamaldehyde can inhibit T1F in S. typhimurium without the growth of bacteria, by regulating the level of expression and transcription of fimA, fimZ, fimY, fimH and fimW. Proteomics results showed that cinnamaldehyde downregulated the subunits and regulators of T1F. In addition, the invasion assays proved that cinnamaldehyde can indeed reduce the ability of S. typhimurium to adhere to cells. The results of animal experiments showed that the colonization in the intestinal tract and the expression levels of inflammatory cytokine were significantly decreased, and the intestinal mucosal immune factors MUC1 and MUC2 were increased under cinnamaldehyde treatment. Therefore, cinnamaldehyde may be a potential drug to target T1F to treat Salmonella infections.

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Published: Nov 10, 2022
Vol/Issue: 27(22)
Pages: 7753
License: View

Authors

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Lizi Yin