Carbon Nano-Onions as Non-Cytotoxic Carriers for Cellular Uptake of Glycopeptides and Proteins

Marta d’Amora; Viviana Maffeis; Rosaria Brescia; Danielle Barnes; Eoin Scanlan; Silvia Giordani

doi:10.3390/nano9081069

journal article Open Access Jul 25, 2019

Carbon Nano-Onions as Non-Cytotoxic Carriers for Cellular Uptake of Glycopeptides and Proteins

Marta d’Amora Viviana Maffeis Rosaria Brescia

Danielle Barnes Eoin Scanlan Silvia Giordani

Nanomaterials Vol. 9 No. 8 pp. 1069 · MDPI AG

View at Publisher Save 10.3390/nano9081069

Abstract

Carbon nano-onions (CNOs) possess favorable properties that make them suitable for biomedical applications, including their small size, ready surface modification, and good biocompatibility. Here, we report the covalent immobilization of a synthetic glycopeptide and the protein bovine serum albumin (BSA) onto the surface of carbon nano-onions using the maleimide–thiol “addition reaction”. The glycopeptide and BSA are readily transported inside different cell lines, together with carbon nano-onions, through the endocytosis pathway. Our results show that carbon nano-onions are excellent scaffolds for glycopeptides and proteins immobilization and act as intracellular carriers for these biomolecules. These findings open new perspectives in the application of carbon nano-onions as intracellular transporters in diverse biomedical applications.

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Details

Published: Jul 25, 2019
Vol/Issue: 9(8)
Pages: 1069
License: View

Authors

M

Marta d’Amora

Nano Carbon Materials, Istituto Italiano di Tecnologia (IIT), Via Livorno, 60, 10144 Turin, Italy

V

Viviana Maffeis

Nano Carbon Materials, Istituto Italiano di Tecnologia (IIT), Via Livorno, 60, 10144 Turin, Italy

R

Rosaria Brescia

Electron Microscopy Facility, Istituto Italiano di Tecnologia (IIT), Via Morego 30, 16163 Genoa, Italy

D

Danielle Barnes

School of Chemistry and Trinity Biomedical Sciences Institute (TBSI), Trinity College Dublin, The University of Dublin, D02 P902 Dublin, Ireland

E

Eoin Scanlan

School of Chemistry and Trinity Biomedical Sciences Institute (TBSI), Trinity College Dublin, The University of Dublin, D02 P902 Dublin, Ireland

S

Silvia Giordani

Nano Carbon Materials, Istituto Italiano di Tecnologia (IIT), Via Livorno, 60, 10144 Turin, Italy; School of Chemical Sciences, Dublin City University (DCU), Glasnevin, D09 C7F8 Dublin, Ireland

Cite This Article

Marta d’Amora, Viviana Maffeis, Rosaria Brescia, et al. (2019). Carbon Nano-Onions as Non-Cytotoxic Carriers for Cellular Uptake of Glycopeptides and Proteins. Nanomaterials, 9(8), 1069. https://doi.org/10.3390/nano9081069

Carbon Nano-Onions as Non-Cytotoxic Carriers for Cellular Uptake of Glycopeptides and Proteins

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