Antihypertensive Effects of Virgin Olive Oil (Unfiltered) Low Molecular Weight Peptides with ACE Inhibitory Activity in Spontaneously Hypertensive Rats

Juan María Alcaide-Hidalgo; Miguel Romero; Juan Duarte; Eduardo López-Huertas

doi:10.3390/nu12010271

journal article Open Access Jan 20, 2020

Antihypertensive Effects of Virgin Olive Oil (Unfiltered) Low Molecular Weight Peptides with ACE Inhibitory Activity in Spontaneously Hypertensive Rats

Juan María Alcaide-Hidalgo

Miguel Romero

Juan Duarte

Eduardo López-Huertas

Nutrients Vol. 12 No. 1 pp. 271 · MDPI AG

View at Publisher Save 10.3390/nu12010271

Abstract

The low molecular weight peptide composition of virgin olive oil (VOO) is mostly unknown. We hypothesised that unfiltered VOO could possess low molecular weight peptides with antihypertensive activity. We produced unfiltered VOO and obtained a water-soluble peptide extract from it. The peptides were separated by size-exclusion using fast protein liquid chromatography, and the low molecular weight fraction was analysed by nanoscale liquid chromatography-Orbitrap coupled with tandem mass spectrometry and de novo sequencing. We selected 23 peptide sequences containing between 6 and 9 amino acids and molecular masses ranging 698–1017 Da. Those peptides were chemically synthesised and their angiotensin-converting enzyme (ACE) inhibitory activity was studied in vitro. Seven peptides showed a strong activity, with half maximal inhibitory concentration (IC50) <10 µm. The antihypertensive effects of the four most active synthesised ACE inhibitor peptides were studied in spontaneously hypertensive rats (SHR). Acute oral administration of synthetic peptides RDGGYCC and CCGNAVPQ showed antihypertensive activity in SHR. We conclude that unfiltered VOO naturally contains low molecular weight peptides with specific ACE inhibitory activity and antihypertensive effects in SHR.

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Details

Published: Jan 20, 2020
Vol/Issue: 12(1)
Pages: 271
License: View

Authors

J

Juan María Alcaide-Hidalgo

Group of Antioxidants and Free Radicals in Biotechnology, Food and Agriculture, Estación Experimental Zaidín, Consejo Superior de Investigaciones Científicas (CSIC), Profesor Albareda 1, 18008 Granada, Spain

M

Miguel Romero

Pharmacology Department, Faculty of Pharmacy, University of Granada, CIBER-Enfermedades Cardiovasculares (CiberCV), 18071 Granada, Spain

J

Juan Duarte

Pharmacology Department, Faculty of Pharmacy, University of Granada, CIBER-Enfermedades Cardiovasculares (CiberCV), 18071 Granada, Spain

E

Eduardo López-Huertas

Group of Antioxidants and Free Radicals in Biotechnology, Food and Agriculture, Estación Experimental Zaidín, Consejo Superior de Investigaciones Científicas (CSIC), Profesor Albareda 1, 18008 Granada, Spain

Funding

European Regional Development Fund Award: recupera2020

Cite This Article

Juan María Alcaide-Hidalgo, Miguel Romero, Juan Duarte, et al. (2020). Antihypertensive Effects of Virgin Olive Oil (Unfiltered) Low Molecular Weight Peptides with ACE Inhibitory Activity in Spontaneously Hypertensive Rats. Nutrients, 12(1), 271. https://doi.org/10.3390/nu12010271

Antihypertensive Effects of Virgin Olive Oil (Unfiltered) Low Molecular Weight Peptides with ACE Inhibitory Activity in Spontaneously Hypertensive Rats

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