Drift of the Subgingival Periodontal Microbiome during Chronic Periodontitis in Type 2 Diabetes Mellitus Patients

Irina P. Balmasova; Evgenii I. Olekhnovich; Ksenia M. Klimina; Anna A. Korenkova; Maria T. Vakhitova; Elmar A. Babaev; Leyla A. Ovchinnikova; Yakov A. Lomakin; Ivan V. Smirnov; Victor N. Tsarev; Ashot M. Mkrtumyan; Alexey A. Belogurov; Alexander G. Gabibov; Elena N. Ilina; Sergey D. Arutyunov

doi:10.3390/pathogens10050504

journal article Open Access Apr 22, 2021

Drift of the Subgingival Periodontal Microbiome during Chronic Periodontitis in Type 2 Diabetes Mellitus Patients

Irina P. Balmasova Evgenii I. Olekhnovich Ksenia M. Klimina Anna A. Korenkova Maria T. Vakhitova Elmar A. Babaev Leyla A. Ovchinnikova

Yakov A. Lomakin Ivan V. Smirnov Victor N. Tsarev Ashot M. Mkrtumyan

Alexey A. Belogurov

Alexander G. Gabibov Elena N. Ilina Sergey D. Arutyunov

Pathogens Vol. 10 No. 5 pp. 504 · MDPI AG

View at Publisher Save 10.3390/pathogens10050504

Abstract

Since periodontitis and type 2 diabetes mellitus are complex diseases, a thorough understanding of their pathogenesis requires knowing the relationship of these pathologies with other disorders and environmental factors. In this study, the representability of the subgingival periodontal microbiome of 46 subjects was studied by 16S rRNA gene sequencing and shotgun sequencing of pooled samples. We examined 15 patients with chronic periodontitis (CP), 15 patients with chronic periodontitis associated with type 2 diabetes mellitus (CPT2DM), and 16 healthy subjects (Control). The severity of generalized chronic periodontitis in both periodontitis groups of patients (CP and CPT2DM) was moderate (stage II). The male to female ratios were approximately equal in each group (22 males and 24 females); the average age of the subjects was 53.9 ± 7.3 and 54.3 ± 7.2 years, respectively. The presence of overweight patients (Body Mass Index (BMI) 30–34.9 kg/m2) and patients with class 1–2 obesity (BMI 35–45.9 kg/m2) was significantly higher in the CPT2DM group than in patients having only chronic periodontitis or in the Control group. However, there was no statistically significant difference in all clinical indices between the CP and CPT2DM groups. An analysis of the metagenomic data revealed that the alpha diversity in the CPT2DM group was increased compared to that in the CP and Control groups. The microbiome biomarkers associated with experimental groups were evaluated. In both groups of patients with periodontitis, the relative abundance of Porphyromonadaceae was increased compared to that in the Control group. The CPT2DM group was characterized by a lower relative abundance of Streptococcaceae/Pasteurellaceae and a higher abundance of Leptotrichiaceae compared to those in the CP and Control groups. Furthermore, the CP and CPT2DM groups differed in terms of the relative abundance of Veillonellaceae (which was decreased in the CPT2DM group compared to CP) and Neisseriaceae (which was increased in the CPT2DM group compared to CP). In addition, differences in bacterial content were identified by a combination of shotgun sequencing of pooled samples and genome-resolved metagenomics. The results indicate that there are subgingival microbiome-specific features in patients with chronic periodontitis associated with type 2 diabetes mellitus.

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Details

Published: Apr 22, 2021
Vol/Issue: 10(5)
Pages: 504
License: View

Authors

I

Irina P. Balmasova

Ministry of Healthcare of the Russian Federation, A.I. Evdokimov Moscow State University of Medicine and Dentistry, 127473 Moscow, Russia

E

Evgenii I. Olekhnovich

Federal Research and Clinical Center of Physical and Chemical Medicine, Federal Medical and Biological Agency of Russia, 119435 Moscow, Russia

K

Ksenia M. Klimina

Federal Research and Clinical Center of Physical and Chemical Medicine, Federal Medical and Biological Agency of Russia, 119435 Moscow, Russia

A

Anna A. Korenkova

Federal Research and Clinical Center of Physical and Chemical Medicine, Federal Medical and Biological Agency of Russia, 119435 Moscow, Russia

M

Maria T. Vakhitova

Federal Research and Clinical Center of Physical and Chemical Medicine, Federal Medical and Biological Agency of Russia, 119435 Moscow, Russia

E

Elmar A. Babaev

Ministry of Healthcare of the Russian Federation, A.I. Evdokimov Moscow State University of Medicine and Dentistry, 127473 Moscow, Russia

L

Leyla A. Ovchinnikova

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS, 117997 Moscow, Russia

Y

Yakov A. Lomakin

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS, 117997 Moscow, Russia

I

Ivan V. Smirnov

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS, 117997 Moscow, Russia; Chemistry Department, Lomonosov Moscow State University, 119991 Moscow, Russia

V

Victor N. Tsarev

Ministry of Healthcare of the Russian Federation, A.I. Evdokimov Moscow State University of Medicine and Dentistry, 127473 Moscow, Russia

A

Ashot M. Mkrtumyan

Ministry of Healthcare of the Russian Federation, A.I. Evdokimov Moscow State University of Medicine and Dentistry, 127473 Moscow, Russia

A

Alexey A. Belogurov

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS, 117997 Moscow, Russia; Chemistry Department, Lomonosov Moscow State University, 119991 Moscow, Russia

A

Alexander G. Gabibov

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS, 117997 Moscow, Russia; Chemistry Department, Lomonosov Moscow State University, 119991 Moscow, Russia; Department of Life Sciences, Higher School of Economics, 101000 Moscow, Russia

E

Elena N. Ilina

Federal Research and Clinical Center of Physical and Chemical Medicine, Federal Medical and Biological Agency of Russia, 119435 Moscow, Russia

S

Sergey D. Arutyunov

Ministry of Healthcare of the Russian Federation, A.I. Evdokimov Moscow State University of Medicine and Dentistry, 127473 Moscow, Russia

Funding

Russian Science Support Foundation Award: 19-14-00331

Cite This Article

Irina P. Balmasova, Evgenii I. Olekhnovich, Ksenia M. Klimina, et al. (2021). Drift of the Subgingival Periodontal Microbiome during Chronic Periodontitis in Type 2 Diabetes Mellitus Patients. Pathogens, 10(5), 504. https://doi.org/10.3390/pathogens10050504

Drift of the Subgingival Periodontal Microbiome during Chronic Periodontitis in Type 2 Diabetes Mellitus Patients

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