Ultrasensitive Ti3C2TX MXene/Chitosan Nanocomposite-Based Amperometric Biosensor for Detection of Potential Prostate Cancer Marker in Urine Samples

Stefania Hroncekova; Tomas Bertok; Michal Hires; Eduard Jane; Lenka Lorencova; Alica Vikartovska; Aisha Tanvir; Peter Kasak; Jan Tkac

doi:10.3390/pr8050580

journal article Open Access May 13, 2020

Ultrasensitive Ti3C2TX MXene/Chitosan Nanocomposite-Based Amperometric Biosensor for Detection of Potential Prostate Cancer Marker in Urine Samples

Stefania Hroncekova Tomas Bertok

Michal Hires Eduard Jane Lenka Lorencova Alica Vikartovska Aisha Tanvir Peter Kasak

Jan Tkac

Processes Vol. 8 No. 5 pp. 580 · MDPI AG

View at Publisher Save 10.3390/pr8050580

Abstract

Two-dimensional layered nanomaterial Ti3C2TX (a member of the MXene family) was used to immobilise enzyme sarcosine oxidase to fabricate a nanostructured biosensor. The device was applied for detection of sarcosine, a potential prostate cancer biomarker, in urine for the first time. The morphology and structures of MXene have been characterised by atomic force microscopy (AFM) and scanning electron microscopy (SEM). Electrochemical measurements, SEM and AFM analysis revealed that MXene interfaced with chitosan is an excellent support for enzyme immobilisation to fabricate a sensitive biosensor exhibiting a low detection limit of 18 nM and a linear range up to 7.8 µM. The proposed biosensing method also provides a short response time of 2 s and high recovery index of 102.6% for detection of sarcosine spiked into urine sample in a clinically relevant range.

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Details

Published: May 13, 2020
Vol/Issue: 8(5)
Pages: 580
License: View

Authors

S

Stefania Hroncekova

Institute of Chemistry, Slovak Academy of Sciences, Dubravska cesta 9, 845 38 Bratislava, Slovak

T

Tomas Bertok

Institute of Chemistry, Slovak Academy of Sciences, Dubravska cesta 9, 845 38 Bratislava, Slovak

M

Michal Hires

Institute of Chemistry, Slovak Academy of Sciences, Dubravska cesta 9, 845 38 Bratislava, Slovak

E

Eduard Jane

Institute of Chemistry, Slovak Academy of Sciences, Dubravska cesta 9, 845 38 Bratislava, Slovak

L

Lenka Lorencova

Institute of Chemistry, Slovak Academy of Sciences, Dubravska cesta 9, 845 38 Bratislava, Slovak

A

Alica Vikartovska

Institute of Chemistry, Slovak Academy of Sciences, Dubravska cesta 9, 845 38 Bratislava, Slovak

A

Aisha Tanvir

Center for Advanced Materials, Qatar University, P. O. BOX 2713, Doha, Qatar

P

Peter Kasak

Center for Advanced Materials, Qatar University, P. O. BOX 2713, Doha, Qatar

J

Jan Tkac

Institute of Chemistry, Slovak Academy of Sciences, Dubravska cesta 9, 845 38 Bratislava, Slovak

Funding

European Research Council Award: 825586

Qatar Foundation Award: NPRP grant # 9 - 219-2-105

Ministry of Health of the Slovak Republic Award: 2019/68-CHÚSAV-1

Cite This Article

Stefania Hroncekova, Tomas Bertok, Michal Hires, et al. (2020). Ultrasensitive Ti3C2TX MXene/Chitosan Nanocomposite-Based Amperometric Biosensor for Detection of Potential Prostate Cancer Marker in Urine Samples. Processes, 8(5), 580. https://doi.org/10.3390/pr8050580

Ultrasensitive Ti3C2TX MXene/Chitosan Nanocomposite-Based Amperometric Biosensor for Detection of Potential Prostate Cancer Marker in Urine Samples

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