The Effect of Aspergillus Thermomutatus Chrysovirus 1 on the Biology of Three Aspergillus Species

Mahjoub A. Ejmal; David J. Holland; Robin M. MacDiarmid; Michael N. Pearson

doi:10.3390/v10100539

journal article Open Access Oct 02, 2018

The Effect of Aspergillus Thermomutatus Chrysovirus 1 on the Biology of Three Aspergillus Species

Mahjoub A. Ejmal David J. Holland Robin M. MacDiarmid

Michael N. Pearson

Viruses Vol. 10 No. 10 pp. 539 · MDPI AG

View at Publisher Save 10.3390/v10100539

Abstract

This study determined the effects of Aspergillus thermomutatus chrysovirus 1 (AthCV1), isolated from Aspergillus thermomutatus, on A. fumigatus, A. nidulans and A. niger. Protoplasts of virus-free isolates of A. fumigatus, A. nidulans and A. niger were transfected with purified AthCV1 particles and the phenotype, growth and sporulation of the isogenic AthCV1-free and AthCV1-infected lines assessed at 20 °C and 37 °C and gene expression data collected at 37 °C. AthCV1-free and AthCV1-infected A. fumigatus produced only conidia at both temperatures but more than ten-fold reduced compared to the AthCV1-infected line. Conidiation was also significantly reduced in infected lines of A. nidulans and A. niger at 37 °C. AthCV1-infected lines of A. thermomutatus and A. nidulans produced large numbers of ascospores at both temperatures, whereas the AthCV1-free line of the former did not produce ascospores. AthCV1-infected lines of all species developed sectoring phenotypes with sclerotia produced in aconidial sectors of A. niger at 37 °C. AthCV1 was detected in 18% of sclerotia produced by AthCV1-infected A. niger and 31% of ascospores from AthCV1-infected A. nidulans. Transcriptome analysis of the naturally AthCV1-infected A. thermomutatus and the three AthCV1-transfected Aspergillus species showed altered gene expression as a result of AthCV1-infection. The results demonstrate that AthCV1 can infect a range of Aspergillus species resulting in reduced sporulation, a potentially useful attribute for a biological control agent.

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Details

Published: Oct 02, 2018
Vol/Issue: 10(10)
Pages: 539
License: View

Authors

M

Mahjoub A. Ejmal

School of Biological Sciences, the University of Auckland, Auckland 1142, New Zealand

D

David J. Holland

Infectious Diseases Unit, Division of Medicine, Middlemore Hospital, Auckland 1640, New Zealand

R

Robin M. MacDiarmid

School of Biological Sciences, the University of Auckland, Auckland 1142, New Zealand; Plant and Food Research, Auckland 1142, New Zealand

M

Michael N. Pearson

School of Biological Sciences, the University of Auckland, Auckland 1142, New Zealand

Funding

Science Faculty, The University of Auckland Award: N/A

Cite This Article

Mahjoub A. Ejmal, David J. Holland, Robin M. MacDiarmid, et al. (2018). The Effect of Aspergillus Thermomutatus Chrysovirus 1 on the Biology of Three Aspergillus Species. Viruses, 10(10), 539. https://doi.org/10.3390/v10100539

The Effect of Aspergillus Thermomutatus Chrysovirus 1 on the Biology of Three Aspergillus Species

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