New Viral Sequences Identified in the Flavescence Dorée Phytoplasma Vector Scaphoideus titanus

Sara Ottati; Marco Chiapello; Luciana Galetto; Domenico Bosco; Cristina Marzachì; Simona Abbà

doi:10.3390/v12030287

journal article Open Access Mar 06, 2020

New Viral Sequences Identified in the Flavescence Dorée Phytoplasma Vector Scaphoideus titanus

Sara Ottati Marco Chiapello Luciana Galetto

Domenico Bosco

Cristina Marzachì

Simona Abbà

Viruses Vol. 12 No. 3 pp. 287 · MDPI AG

View at Publisher Save 10.3390/v12030287

Abstract

(1) Background: The leafhopper Scaphoideus titanus is the primary vector of Flavescence dorée phytoplasma (FDp) in European vineyards. Flavescence dorée is one of the most severely damaging diseases of Vitis vinifera and, consequently, a major threat to grape and wine production in several European countries. Control measures are compulsory, but they mainly involve large-scale insecticide treatments, with detrimental impacts on the environment. One possible solution is to exploit the largely unexplored genetic diversity of viruses infecting S. titanus as highly specific and environmentally benign tools for biological control. (2) Methods: A metatranscriptomic approach was adopted to identify viruses that may infect individuals caught in the wild in both its native (United States) and invasive (Europe) areas. Reverse transcription PCR was used to confirm their presence in RNA pools and explore their prevalence. (3) Results: We described nine new RNA viruses, including members of “Picorna-Calici”, “Permutotetra”, “Bunya-Arena”, “Reo”, “Partiti-Picobirna”, “Luteo-Sobemo” and “Toti-Chryso” clades. A marked difference in the diversity and abundance of the viral species was observed between the USA population and the European ones. (4) Conclusions: This work represents the first survey to assess the viral community of a phytoplasma insect vector. The possibility to exploit these naturally occurring viruses as specific and targeted biocontrol agents of S. titanus could be the answer to increasing demand for a more sustainable viticulture.

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Details

Published: Mar 06, 2020
Vol/Issue: 12(3)
Pages: 287
License: View

Authors

S

Sara Ottati

Dipartimento di Scienze Agrarie, Forestali ed Alimentari, DISAFA, Università degli Studi di Torino, Largo Paolo Braccini 2, I-10095 Grugliasco, Italy; Consiglio Nazionale delle Ricerche—Istituto per la Protezione Sostenibile delle Piante (CNR-IPSP), Strada delle Cacce 73, I-10135 Torino, Italy

M

Marco Chiapello

Consiglio Nazionale delle Ricerche—Istituto per la Protezione Sostenibile delle Piante (CNR-IPSP), Strada delle Cacce 73, I-10135 Torino, Italy

L

Luciana Galetto

Consiglio Nazionale delle Ricerche—Istituto per la Protezione Sostenibile delle Piante (CNR-IPSP), Strada delle Cacce 73, I-10135 Torino, Italy

D

Domenico Bosco

Dipartimento di Scienze Agrarie, Forestali ed Alimentari, DISAFA, Università degli Studi di Torino, Largo Paolo Braccini 2, I-10095 Grugliasco, Italy; Consiglio Nazionale delle Ricerche—Istituto per la Protezione Sostenibile delle Piante (CNR-IPSP), Strada delle Cacce 73, I-10135 Torino, Italy

C

Cristina Marzachì

Consiglio Nazionale delle Ricerche—Istituto per la Protezione Sostenibile delle Piante (CNR-IPSP), Strada delle Cacce 73, I-10135 Torino, Italy

S

Simona Abbà

Consiglio Nazionale delle Ricerche—Istituto per la Protezione Sostenibile delle Piante (CNR-IPSP), Strada delle Cacce 73, I-10135 Torino, Italy

Funding

European Commission Award: 774208

Cite This Article

Sara Ottati, Marco Chiapello, Luciana Galetto, et al. (2020). New Viral Sequences Identified in the Flavescence Dorée Phytoplasma Vector Scaphoideus titanus. Viruses, 12(3), 287. https://doi.org/10.3390/v12030287

New Viral Sequences Identified in the Flavescence Dorée Phytoplasma Vector Scaphoideus titanus

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