Occurrence, Distribution, and Transmission of Alfalfa Viruses in China

Jin Li; Qiaoxia Shang; Yanqi Liu; Wenting Dai; Xin Li; Shuhua Wei; Guixin Hu; Mark Richard McNeill; Liping Ban

doi:10.3390/v14071519

journal article Open Access Jul 12, 2022

Occurrence, Distribution, and Transmission of Alfalfa Viruses in China

Jin Li

Qiaoxia Shang Yanqi Liu

Wenting Dai

Xin Li

Shuhua Wei Guixin Hu Mark Richard McNeill

Liping Ban

Viruses Vol. 14 No. 7 pp. 1519 · MDPI AG

View at Publisher Save 10.3390/v14071519

Abstract

Alfalfa (Medicago sativa L.) is one of the most important quality forages worldwide and is cultivated throughout China. Alfalfa is susceptible to a variety of viral diseases during its growth, which has caused huge amounts of commercial losses. However, the profile of the alfalfa virus in China remains ambiguous and the viruses transmitted by Odontothrips loti (Haliday), dominant insect pests in alfalfa, are also poorly understood. In the present study, virus diversity was investigated in the primary alfalfa-growing areas in China. A total of 18 alfalfa viruses were identified through RNA-sequencing (RNA-seq) and reverse transcription-polymerase chain reaction (RT-PCR). Two new plant viruses, Medicago sativa virus 1 (MsV1) and Medicago sativa luteovirus 1 (MsLV1), were detected for the first time. Another four viruses, including the Alfalfa ringspot-associated virus (ARaV), Alfalfa virus F (AVF), Alfalfa enamovirus 1 (AEV1), and Alfalfa deltaparitivirus (ADPV), were reported in China for the first time as well. Both Alfalfa mosaic virus (AMV) and Medicago sativa alphapartitivirus 2 (MsAPV2) are the dominant pathogens, with an infection incidence of 91.7–100%, and 74.4–97.2%, respectively. Additionally, O. loti with first- and second-instar nymphs were shown to acquire the AMV within 0.25 h of feeding on a virus-infected alfalfa. Transmission by thrips to healthy alfalfa plants was also demonstrated. Additionally, we clarified the dynamic changes in the AMV in pre-adult stages of O. loti, which indicated that the AMV is propagated in the nymph stage of O. loti. These findings provide valuable information for understanding the alfalfa virome, confirm the role thrips O. loti plays in alfalfa virus transmission, and improve our fundamental knowledge and management of diseases in China.

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Details

Published: Jul 12, 2022
Vol/Issue: 14(7)
Pages: 1519
License: View

Authors

J

Jin Li

College of Grassland Science and Technology, China Agricultural University, Beijing 100193, China

Q

Qiaoxia Shang

College of Bioscience and Resources Environment, Beijing University of Agriculture, Beijing 100096, China

Y

Yanqi Liu

College of Grassland Science and Technology, China Agricultural University, Beijing 100193, China

W

Wenting Dai

College of Grassland Science and Technology, China Agricultural University, Beijing 100193, China

X

Xin Li

College of Grassland Science and Technology, China Agricultural University, Beijing 100193, China

S

Shuhua Wei

Institute of Plant Protection, Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan 750002, China

G

Guixin Hu

Pratacultural College, Gansu Agricultural University, Lanzhou 730070, China

M

Mark Richard McNeill

Resilient Agriculture Innovative Centre of Excellence, AgResearch, Ltd., Lincoln 7674, New Zealand

L

Liping Ban

College of Grassland Science and Technology, China Agricultural University, Beijing 100193, China

Funding

National Natural Science Foundation of China (NSFC) Award: 31971759

Beijing Food Crops Innovation Consortium Award: 31971759

Cite This Article

Jin Li, Qiaoxia Shang, Yanqi Liu, et al. (2022). Occurrence, Distribution, and Transmission of Alfalfa Viruses in China. Viruses, 14(7), 1519. https://doi.org/10.3390/v14071519

Occurrence, Distribution, and Transmission of Alfalfa Viruses in China

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