Flaviviral Replication Complex: Coordination between RNA Synthesis and 5’-RNA Capping

Valerie Klema; Radhakrishnan Padmanabhan; Kyung Choi

doi:10.3390/v7082837

journal article Open Access Aug 13, 2015

Flaviviral Replication Complex: Coordination between RNA Synthesis and 5’-RNA Capping

Valerie Klema Radhakrishnan Padmanabhan Kyung Choi

Viruses Vol. 7 No. 8 pp. 4640-4656 · MDPI AG

View at Publisher Save 10.3390/v7082837

Abstract

Genome replication in flavivirus requires (—) strand RNA synthesis, (+) strand RNA synthesis, and 5’-RNA capping and methylation. To carry out viral genome replication, flavivirus assembles a replication complex, consisting of both viral and host proteins, on the cytoplasmic side of the endoplasmic reticulum (ER) membrane. Two major components of the replication complex are the viral non-structural (NS) proteins NS3 and NS5. Together they possess all the enzymatic activities required for genome replication, yet how these activities are coordinated during genome replication is not clear. We provide an overview of the flaviviral genome replication process, the membrane-bound replication complex, and recent crystal structures of full-length NS5. We propose a model of how NS3 and NS5 coordinate their activities in the individual steps of (—) RNA synthesis, (+) RNA synthesis, and 5’-RNA capping and methylation.

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Details

Published: Aug 13, 2015
Vol/Issue: 7(8)
Pages: 4640-4656
License: View

Authors

V

Valerie Klema

Department of Biochemistry and Molecular Biology, Sealy Center for Structural Biology and Molecular Biophysics, University of Texas Medical Branch at Galveston, Galveston, TX 77555-0647, USA

R

Radhakrishnan Padmanabhan

Department of Microbiology and Immunology, Georgetown University School of Medicine, Washington, DC 20057, USA

K

Kyung Choi

Department of Biochemistry and Molecular Biology, Sealy Center for Structural Biology and Molecular Biophysics, University of Texas Medical Branch at Galveston, Galveston, TX 77555-0647, USA

Cite This Article

Valerie Klema, Radhakrishnan Padmanabhan, Kyung Choi (2015). Flaviviral Replication Complex: Coordination between RNA Synthesis and 5’-RNA Capping. Viruses, 7(8), 4640-4656. https://doi.org/10.3390/v7082837

Flaviviral Replication Complex: Coordination between RNA Synthesis and 5’-RNA Capping

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