journal article Open Access Feb 01, 2017

Twenty microsatellite markers for the endangered Vatica mangachapoi (Dipterocarpaceae)

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Abstract
Premise of the study:Microsatellite markers were developed for Vatica mangachapoi (Dipterocarpaceae), an endangered species indigenous to Southeast Asia and southern China.Methods and Results:Twenty microsatellite markers, including 12 polymorphic markers, were identified from V. mangachapoi using high‐throughput sequencing. Polymorphism at each marker was evaluated using 87 individuals from three natural populations. The number of alleles per polymorphic locus ranged from six to 15, and the observed and expected heterozygosity varied from 0.000 to 0.926 and from 0.177 to 0.864, respectively. These markers were transferred successfully to the endangered species V. guangxiensis.Conclusions:These markers may be used to investigate the genetic diversity and gene flow of V. mangachapoi and V. guangxiensis.
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References
11
[1]
Appanah S. (1998)
[2]
Huang J. X. "Genetic diversity of Vatica mangachapoi in Hainan Island revealed by AFLP" Scientia Silvae Sinicae (2008)
[3]
IUCN.2014.The IUCN Red List of Threatened Species. Version 2014.3. Websitehttp://www.iucnredlist.org[accessed 16 March 2015].
[5]
Li X. W. (2007)
[8]
micro‐checker: software for identifying and correcting genotyping errors in microsatellite data

COCK VAN OOSTERHOUT, WILLIAM F. HUTCHINSON, DEREK P. M. WILLS et al.

Molecular Ecology Notes 10.1111/j.1471-8286.2004.00684.x
[9]
Wang S. (2004)
[10]
Yeh F. C. (1999)
[11]
Zeng J. "Preparation of total DNA from ‘recalcitrant plant taxa" Acta Botanica Sinica (2002)
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Published
Feb 01, 2017
Vol/Issue
5(2)
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Cite This Article
Jun‐Jie Guo, Shuai‐Bin Shang, Chun‐Sheng Wang, et al. (2017). Twenty microsatellite markers for the endangered Vatica mangachapoi (Dipterocarpaceae). Applications in Plant Sciences, 5(2). https://doi.org/10.3732/apps.1600134