Monoclonal antibodies capable of causing hemolysis of neuraminidase-treated human erythrocytes by homologous complement.

N Okada; R Harada; T Fujita; H Okada

doi:10.4049/jimmunol.143.7.2262

journal article Oct 01, 1989

Monoclonal antibodies capable of causing hemolysis of neuraminidase-treated human erythrocytes by homologous complement.

N Okada R Harada T Fujita H Okada

The Journal of Immunology Vol. 143 No. 7 pp. 2262-2266 · Oxford University Press (OUP)

View at Publisher Save 10.4049/jimmunol.143.7.2262

Abstract

Abstract
As human E (HuE) treated with neuraminidase (Neu) are resistant to hemolysis by human serum but are readily lysed by heterologous serum via the alternative C pathway, we attempted to produce mAb which might modify Neu-treated HuE (Neu-HuE) so as to render them sensitive to homologous C. A hybridoma, clone -1F5, was obtained by screening for antibody which caused hemolysis of Neu-HuE by human serum via the alternative C pathway. We have shown that this antibody (1F5) of IgG1 isotype blocks the action of a 20-kDa membrane inhibitor capable of interfering with the terminal step in the homologous C cascade. The antigenic molecule can be termed HRF20, which stands for homologous restriction factor (HRF) with m.w. 20,000, because its function is essentially the same as that of HRF (68,000) reported by others.

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Details

Published: Oct 01, 1989
Vol/Issue: 143(7)
Pages: 2262-2266
License: View

Authors

N

N Okada

Department of Microbiology, Fukuoka University School of Medicine , Fukuoka 814-01

R

R Harada

Department of Microbiology, Fukuoka University School of Medicine , Fukuoka 814-01

T

T Fujita

Department of Immunology, Tsukuba University School of Medicine , Tsukuba 305

H

H Okada

Department of Microbiology, Fukuoka University School of Medicine , Fukuoka 814-01; Department of Molecular Biology, Nagoya City University School of Medicine , Nagoya 467 ,

Cite This Article

N Okada, R Harada, T Fujita, et al. (1989). Monoclonal antibodies capable of causing hemolysis of neuraminidase-treated human erythrocytes by homologous complement.. The Journal of Immunology, 143(7), 2262-2266. https://doi.org/10.4049/jimmunol.143.7.2262

Monoclonal antibodies capable of causing hemolysis of neuraminidase-treated human erythrocytes by homologous complement.

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