Porcine gelatine detection &lt;i&gt;via &lt;/i&gt;electrochemical immunosensors utilizing green-synthesized cerium oxide nanoparticles from orange peel

Maintaining the safety and proper labelling of food products is vital for communities around the world. One of the primary concerns in food safety is the unintentional presence of porcine derivatives such as gelatine in food products. This issue could affect the labelling accuracy of food products, religious dietary observances, regulatory compliance, and public health. Electrochemical immunosensors address this issue by rapidly, portably, and especially with high sensitivity, detecting porcine gelatine. The electrode was modified with CeO2 nanoparticles (NPs) synthesized via a green method using orange peel extract, which enhanced the immobilization of the bioreceptor on the electrode surface. Orange peel extract contains secondary metabolites that can act as stabilizing agents and prevent agglomeration during the synthesis process. The synthesized CeO2 NPs show a cube morphology, producing an average particle size of 12 nm with 77.31 % crystallinity. The performance of green synthesized CeO2 NPs modified electrode are successful for detecting porcine gelatine in solution and commercialized gelatine product, resulting in a limit of detection of 3.53 ppm. These results show the potential of green-synthesized CeO2 NPs for an electrochemical immunosensor for detecting porcine products.

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Details

Published: Jul 31, 2025
Vol/Issue: 15(5)
License: View

Authors

Biyas Aurora Nania Nevada

E

Elisabeth Nasya Dominika Setyawati Putri

S

Salma Nur Zakiyyah

Y

Yohanes Susanto Ridwan

Funding

Kementerian Riset Teknologi Dan Pendidikan Tinggi Republik Indonesia Award: PKM 2024

Universitas Padjadjaran Award: Contract no. 1666/UN6.3.1/PT.00/2024

Cite This Article

Rafa Radithya Swara, Nazwa Alya Zahra, Dea Hasna Tsary, et al. (2025). Porcine gelatine detection <i>via </i>electrochemical immunosensors utilizing green-synthesized cerium oxide nanoparticles from orange peel. Journal of Electrochemical Science and Engineering, 15(5). https://doi.org/10.5599/jese.2698

Porcine gelatine detection &lt;i&gt;via &lt;/i&gt;electrochemical immunosensors utilizing green-synthesized cerium oxide nanoparticles from orange peel

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Porcine gelatine detection <i>via </i>electrochemical immunosensors utilizing green-synthesized cerium oxide nanoparticles from orange peel