Effects of dietary β-mannanase supplementation in low-energy and low-protein diets containing high-mannan ingredients on growth performance, meat quality, intestinal morphology, and liver health in broiler chickens

Eun Cheol Lee; Kang Hyeon Kim; Charline Mugeniwayesu; Ye Jin Jeong; Dong Yong Kil

doi:10.5713/ab.250545

journal article Open Access Apr 01, 2026

Effects of dietary β-mannanase supplementation in low-energy and low-protein diets containing high-mannan ingredients on growth performance, meat quality, intestinal morphology, and liver health in broiler chickens

Eun Cheol Lee

Kang Hyeon Kim

Charline Mugeniwayesu

Ye Jin Jeong

Dong Yong Kil

Animal Bioscience Vol. 39 No. 4 pp. 250545 · Asian Australasian Association of Animal Production Societies

View at Publisher Save 10.5713/ab.250545

Abstract

Objective: This study was conducted to investigate the effect of dietary β-mannanase (MN) supplementation in low-energy and low-protein diets containing high-mannan ingredients on growth performance, meat quality, intestinal morphology, and liver health in broiler chickens.Methods: A total of 1,120 1-d-old Ross 308 broiler chickens were allotted to 1 of 5 dietary treatments. The positive control (PC) diet was prepared with corn and soybean meal, whereas the negative control (NC) diet was formulated with decreased AMEn by 100 kcal/kg and CP by approximately 1.0% than PC diets. High-mannan NC diet was also prepared by inclusion of 2.5% palm kernel meal (PKM) and 2.5% copra meal (CM), which contained energy and nutrient concentrations equal to those in the NC diet. Finally, dietary MN was supplemented to the high-mannan NC diet at 2 different levels of 0.05% and 0.10%.Results: Broiler performance was impaired (p<0.05) by feeding low-energy and low-protein diets, regardless of inclusion of PKM and CM. No positive effects of dietary MN supplementation in low-energy and low-protein diets containing PKM and CM on broiler performance were observed. Inclusion of PKM and CM increased (p<0.05) jejunal digesta viscosity but 0.10% MN supplementation decreased (p<0.05) jejunal digesta viscosity. Likewise, a tendency (p = 0.056) for increased villus height:crypt depth ratio was observed by 0.05% or 0.10% MN supplementation. However, dietary treatments had no effects on liver health in broiler chickens.Conclusion: Feeding low-energy and low-protein diets with reduction in 100 kcal/kg AMEn and approximately 1.0% CP impaired broiler performance. Inclusion of 2.5% PKM and 2.5% CM with or without MN supplementation had no considerable effects on broiler performance. However, MN supplementation in low-energy and low-protein diets containing PKM and CM decreased digesta viscosity with improving jejunal morphology.

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Details

Published: Apr 01, 2026
Vol/Issue: 39(4)
Pages: 250545
License: View

Authors

E

Eun Cheol Lee

Center for Superfunctional Materials, Department of Chemistry, Pohang University of Science and Technology, San 31, Hyojadong, Namgu, Pohang 790-784, Korea, Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Flemingovo, n. 2. Praha 6 166 10, Czech Republic, and School of Computational Science, Korea Institute for Advanced Study, 207-43, Cheongyangni-2-dong, Dongdaemun-gu, Seoul 130-722, Korea

K

Kang Hyeon Kim

C

Charline Mugeniwayesu

Funding

Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries

Chung-Ang University

Cite This Article

Eun Cheol Lee, Kang Hyeon Kim, Charline Mugeniwayesu, et al. (2026). Effects of dietary β-mannanase supplementation in low-energy and low-protein diets containing high-mannan ingredients on growth performance, meat quality, intestinal morphology, and liver health in broiler chickens. Animal Bioscience, 39(4), 250545. https://doi.org/10.5713/ab.250545

Effects of dietary β-mannanase supplementation in low-energy and low-protein diets containing high-mannan ingredients on growth performance, meat quality, intestinal morphology, and liver health in broiler chickens

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