The AUX1-AFB1-CNGC14 module establishes a longitudinal root surface pH profile

Nelson BC Serre; Daša Wernerová; Pruthvi Vittal; Shiv Mani Dubey; Eva Medvecká; Adriana Jelínková; Jan Petrášek; Guido Grossmann; Matyáš Fendrych

doi:10.7554/elife.85193

journal article Open Access Jul 14, 2023

The AUX1-AFB1-CNGC14 module establishes a longitudinal root surface pH profile

Nelson BC Serre Daša Wernerová Pruthvi Vittal Shiv Mani Dubey Eva Medvecká Adriana Jelínková Jan Petrášek

Guido Grossmann

Matyáš Fendrych

eLife Vol. 12 · eLife Sciences Publications, Ltd

View at Publisher Save 10.7554/elife.85193

Abstract

Plant roots navigate in the soil environment following the gravity vector. Cell divisions in the meristem and rapid cell growth in the elongation zone propel the root tips through the soil. Actively elongating cells acidify their apoplast to enable cell wall extension by the activity of plasma membrane AHA H
+
-ATPases. The phytohormone auxin, central regulator of gravitropic response and root development, inhibits root cell growth, likely by rising the pH of the apoplast. However, the role of auxin in the regulation of the apoplastic pH gradient along the root tip is unclear. Here, we show, by using an improved method for visualization and quantification of root surface pH, that the
Arabidopsis thaliana
root surface pH shows distinct acidic and alkaline zones, which are not primarily determined by the activity of AHA H
+
-ATPases. Instead, the distinct domain of alkaline pH in the root transition zone is controlled by a rapid auxin response module, consisting of the AUX1 auxin influx carrier, the AFB1 auxin co-receptor, and the CNCG14 calcium channel. We demonstrate that the rapid auxin response pathway is required for an efficient navigation of the root tip.

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Details

Published: Jul 14, 2023
Vol/Issue: 12
License: View

Authors

N

Nelson BC Serre

Department of Experimental Plant Biology, Faculty of Science, Charles University

D

Daša Wernerová

Department of Experimental Plant Biology, Faculty of Science, Charles University; Institute of Cell and Interaction Biology, Heinrich-Heine-University Düsseldorf

P

Pruthvi Vittal

Department of Experimental Plant Biology, Faculty of Science, Charles University

S

Shiv Mani Dubey

Department of Experimental Plant Biology, Faculty of Science, Charles University

E

Eva Medvecká

Department of Experimental Plant Biology, Faculty of Science, Charles University

A

Adriana Jelínková

Institute of Experimental Botany, Czech Academy of Sciences

J

Jan Petrášek

Department of Experimental Plant Biology, Faculty of Science, Charles University; Institute of Experimental Botany, Czech Academy of Sciences

G

Guido Grossmann

Institute of Cell and Interaction Biology, Heinrich-Heine-University Düsseldorf; CEPLAS - Cluster of Excellence on Plant Sciences, Heinrich-Heine-University Düsseldorf

M

Matyáš Fendrych

Department of Experimental Plant Biology, Faculty of Science, Charles University

Funding

Deutsche Forschungsgemeinschaft Award: GR4559/4-1

European Research Council Award: 803048

CEPLAS-EXC-2048/1 Award: 390686111

Cite This Article

Nelson BC Serre, Daša Wernerová, Pruthvi Vittal, et al. (2023). The AUX1-AFB1-CNGC14 module establishes a longitudinal root surface pH profile. eLife, 12. https://doi.org/10.7554/elife.85193

The AUX1-AFB1-CNGC14 module establishes a longitudinal root surface pH profile

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