Marked variations in gut microbiota and some innate immune responses of fresh water crayfish, marron (
                    Cherax cainii
                    , Austin 2002) fed dietary supplementation of
                    Clostridium butyricum

Md Javed Foysal; Thi Thu Thuy Nguyen; Md Reaz Chaklader; Muhammad A.B. Siddik; Chin-Yen Tay; Ravi Fotedar

doi:10.7717/peerj.7553

journal article Open Access Aug 27, 2019

Marked variations in gut microbiota and some innate immune responses of fresh water crayfish, marron ( Cherax cainii , Austin 2002) fed dietary supplementation of Clostridium butyricum

Md Javed Foysal

Thi Thu Thuy Nguyen Md Reaz Chaklader

Muhammad A.B. Siddik Chin-Yen Tay Ravi Fotedar

PeerJ Vol. 7 pp. e7553 · PeerJ

View at Publisher Save 10.7717/peerj.7553

Abstract

This study aimed to investigate the effects of
Clostridium butyricum
as a dietary probiotic supplement in fishmeal based diet on growth, gut microbiota and immune performance of marron (
Cherax cainii
). Marron were randomly distributed into two different treatment groups, control and probiotic fed group. After 42 days of feeding trial, the results revealed a significant (
P
< 0.05) increase in growth due to increase in number of moults in marron fed probiotics. The probiotic diet also significantly enhanced the total haemocyte counts (THC), lysozyme activity in the haemolymph and protein content of the tail muscle in marron. Compared to control, the 16S rRNA sequences data demonstrated an enrichment of bacterial diversity in the probiotic fed marron where significant increase of
Clostridium
abundance was observed. The abundance for crayfish pathogen
Vibrio
and
Aeromonas
were found to be significantly reduced post feeding with probiotic diet. Predicted metabolic pathway revealed an increased activity for the metabolism and absorption of carbohydrate, degradation of amino acid, fatty acid and toxic compounds, and biosynthesis of secondary metabolites.
C. butyricum
supplementation also significantly modulated the expression level of immune-responsive genes of marron post challenged with
Vibrio mimicus
. The overall results suggest that
C. butyricum
could be used as dietary probiotic supplement in marron aquaculture.

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Details

Published: Aug 27, 2019
Vol/Issue: 7
Pages: e7553
License: View

Authors

M

Md Javed Foysal

School of Molecular and Life Sciences, Curtin University, Bentley, Western Australia, Australia; Department of Genetic Engineering and Biotechnology, Shahjalal University of Science & Technology, Sylhet, Bangladesh

T

Thi Thu Thuy Nguyen

School of Molecular and Life Sciences, Curtin University, Bentley, Western Australia, Australia

M

Md Reaz Chaklader

School of Molecular and Life Sciences, Curtin University, Bentley, Western Australia, Australia

M

Muhammad A.B. Siddik

School of Molecular and Life Sciences, Curtin University, Bentley, Western Australia, Australia; Department of Fisheries Biology and Genetics, Patuakhali Science and Technology University, Patuakhali, Bangladesh

C

Chin-Yen Tay

Helicobacter Research Laboratory, Marshall Centre for Infectious Disease Research and Training, School of Biomedical Sciences, University of Western Australia, Perth, Western Australia, Australia

R

Ravi Fotedar

School of Molecular and Life Sciences, Curtin University, Bentley, Western Australia, Australia

Funding

Australian Government, Department of Education and Training

Cite This Article

Md Javed Foysal, Thi Thu Thuy Nguyen, Md Reaz Chaklader, et al. (2019). Marked variations in gut microbiota and some innate immune responses of fresh water crayfish, marron ( Cherax cainii , Austin 2002) fed dietary supplementation of Clostridium butyricum. PeerJ, 7, e7553. https://doi.org/10.7717/peerj.7553

Marked variations in gut microbiota and some innate immune responses of fresh water crayfish, marron ( Cherax cainii , Austin 2002) fed dietary supplementation of Clostridium butyricum

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