Differences of Viral Hemorrhagic Septicemia Virus Loads among Organs of Dead and Surviving Olive Flounder Infected by Intramuscular Injection and Immersion Challenge

Soo-Jin Kim; Wi-Sik Kim; Myung‐Joo Oh

doi:10.1002/aah.10068

journal article May 08, 2019

Differences of Viral Hemorrhagic Septicemia Virus Loads among Organs of Dead and Surviving Olive Flounder Infected by Intramuscular Injection and Immersion Challenge

Soo-Jin Kim Wi-Sik Kim Myung‐Joo Oh

Journal of Aquatic Animal Health Vol. 31 No. 2 pp. 193-200 · Oxford University Press (OUP)

View at Publisher Save 10.1002/aah.10068

Abstract

Abstract
Viral hemorrhagic septicemia virus (VHSV) is an important viral pathogen in the culture of Olive Flounder Paralichthys olivaceus. Based on cumulative mortality, the virulence of VHSV was found to be highly different depending on challenge routes and exposure doses (using tissue culture infectious dose with 50% endpoint [TCID50]). Olive Flounder were injected with VHSV at 102.5, 104.5, 106.5, and 108.5 TCID50/100 μL/fish. A second group of fish was immersed at 103.5, 105.5, and 107.5 TCID50/mL at 10°C for 1 h in this study. The cumulative mortality was observed at 15 d postinfection. Immersion challenge at 103.5 TCID50/mL caused no mortality, while intramuscular injection challenge resulted in high levels of mortality with all VHSV exposure doses. Overall, Olive Flounder was susceptible to VHSV, with cumulative mortality of 90% or 100% in fish intramuscularly injected with high or low doses of VHSV. The cumulative mortality was 40% and 70% at 105.5 and 107.5 TCID50/mL, respectively, in the immersion challenge group. The VHSV titration and copy numbers were estimated by TCID50 and quantitative reverse transcription PCR methods. From dead Olive Flounder, VHSV titration was consistently detected in all tested organs, ranging from 105 to 109 TCID50/mL. The VHSV titration was under the detection limit from surviving Olive Flounder, but the VHSV N gene was detected.

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Metrics

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References

Details

Published: May 08, 2019
Vol/Issue: 31(2)
Pages: 193-200
License: View

Authors

S

Soo-Jin Kim

Department of Aqualife Medicine, Chonnam National University, Yeosu, 59626, South Korea

W

Wi-Sik Kim

Department of Aqualife Medicine, Chonnam National University, Yeosu, 59626, South Korea

M

Myung‐Joo Oh

Department of Aqualife Medicine, Chonnam National University, Yeosu, 59626, South Korea

Funding

National Research Foundation Award: 2016R1D1A3B-03930749

Chonnam National University

Cite This Article

Soo-Jin Kim, Wi-Sik Kim, Myung‐Joo Oh (2019). Differences of Viral Hemorrhagic Septicemia Virus Loads among Organs of Dead and Surviving Olive Flounder Infected by Intramuscular Injection and Immersion Challenge. Journal of Aquatic Animal Health, 31(2), 193-200. https://doi.org/10.1002/aah.10068

Differences of Viral Hemorrhagic Septicemia Virus Loads among Organs of Dead and Surviving Olive Flounder Infected by Intramuscular Injection and Immersion Challenge

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