Encapsulation of plant growth‐promoting bacteria in alginate beads enriched with humic acid

Chiu‐Chung Young; P.D. Rekha; Wei‐An Lai; A.B. Arun

doi:10.1002/bit.20957

journal article Apr 17, 2006

Encapsulation of plant growth‐promoting bacteria in alginate beads enriched with humic acid

Chiu‐Chung Young P.D. Rekha Wei‐An Lai A.B. Arun

Biotechnology and Bioengineering Vol. 95 No. 1 pp. 76-83 · Wiley

View at Publisher Save 10.1002/bit.20957

Abstract

AbstractThe key to achieving successful, reproducible results following the introduction of beneficial microbes into soil relies on the survival rate of the inoculated bacteria in a heterogeneous soil environment and hence an improved encapsulation method was developed. Owing to the constraints associated with the inoculum formulation, in this study, encapsulation of a plant growth promoting bacteria (PGPB) isolate Bacillus subtilis CC‐pg104 was attempted with alginate by enriching the bead microenvironment with humic acid. High viability of the encapsulated bacteria was observed with minimum cell loss upon storage for 5 months. Steady and constant cell release from the bead was observed for 1 week at different pH. Encapsulated cells remained active as evidenced by their ability to solubilize calcium phosphate in vitro. Successful plant growth promotion of lettuce by the encapsulated bacteria under gnotobiotic and sterile environment was also achieved. Feasibility of this improved encapsulation technique is mainly due to the dual benefits of humic acid to microbe and plant and its chemical properties allowing an easy mixing with alginate without interfering in the formation of the alginate gel beads by cross‐linking with Ca2+ ions. Thus, the encapsulation method described in this study can be effectively used to protect the PGPB inoculum from adverse conditions of the soil for their successful establishment in the rhizosphere. © 2006 Wiley Periodicals, Inc.

Topics

No keywords indexed for this article. Browse by subject →

References

40

[1]

10.1007/s00374-003-0650-1

[2]

10.2135/cropsci2003.1774

[3]

Bashan Y "Alginate beads as synthetic inoculant carriers for the slow release of bacteria that affect plant growth" Appl Environ Microbiol (1986) 10.1128/aem.51.5.1089-1098.1986

[4]

10.1016/0038-0717(86)90064-7

[5]

10.1016/s0734-9750(98)00003-2

[6]

10.1016/b0-12-348530-4/00513-0

[7]

10.1007/s002530051391

[8]

10.1007/s00374-002-0481-5

[9]

10.1016/s1369-5266(00)00183-7

[10]

10.1016/s0065-2113(08)60425-3

[11]

10.1007/bf01570068

[12]

10.1016/s0167-7012(97)00059-6

[13]

10.2136/sssaj1999.6361670x

[14]

10.1002/(sici)1097-0290(19960720)51:2<157::aid-bit4>3.0.co;2-i

[15]

Chanway CP (2002)

[16]

10.1016/s0960-8524(99)00150-9

[17]

The enhancement of plant growth by free-living bacteria

Bernard R. Glick

Canadian Journal of Microbiology 10.1139/m95-015

[18]

HoglandDR ArnonD.1950.The water culture method for growing plants without soil. California Agriculture Experiment station circular No. 347: p39.

[19]

Differential Ability of Genotypes of 2,4-Diacetylphloroglucinol-Producing Pseudomonas fluorescens Strains To Colonize the Roots of Pea Plants

Blanca B. Landa, Olga V. Mavrodi, Jos M. Raaijmakers et al.

Applied and Environmental Microbiology 10.1128/aem.68.7.3226-3237.2002

[20]

10.1023/b:anto.0000024903.10757.6e

[21]

10.1128/aem.64.7.2341-2345.1998

[22]

Physiological effects of humic substances on higher plants

Serenella Nardi, Diego Pizzeghello, Adele Muscolo et al.

Soil Biology and Biochemistry 10.1016/s0038-0717(02)00174-8

[23]

An efficient microbiological growth medium for screening phosphate solubilizing microorganisms

C.Shekhar Nautiyal

FEMS Microbiology Letters 10.1111/j.1574-6968.1999.tb13383.x

[24]

10.2307/2401670

[25]

10.1016/s0734-9750(00)00040-9

[26]

10.1016/j.tplants.2004.04.008

[27]

10.1093/jxb/erh085

[28]

10.1094/mpmi-9-0600

[29]

10.1016/s0142-9612(03)00418-6

[30]

10.1016/0167-7799(90)90139-o

[31]

10.1007/bf00167859

[32]

Stat Soft Inc.1995.STATISTICA for windows (computer program manual). Tulsa OK: Stat Soft Inc. 2325 East 13th Street Tulsa OK 74 104.

[33]

Tang KH (1994)

[34]

10.1007/bf00205067

[35]

10.1016/s0168-1656(01)00341-8

[36]

10.1016/s0944-5013(00)80033-6

[37]

10.1007/bf02431932

[38]

Witter L (1996)

[39]

Young CC "Prospects for nitrogen incorporation into humic acid as evidenced by alkaline extraction method" Curr Sci (2004)

[40]

10.1021/bp025532t

Metrics

153

Citations

40

References

Details

Published: Apr 17, 2006
Vol/Issue: 95(1)
Pages: 76-83
License: View

Authors

Department of Pathology Kaohsiung Medical University Hospital, Kaohsiung Medical University Kaohsiung Taiwan

A

A.B. Arun

Cite This Article

Chiu‐Chung Young, P.D. Rekha, Wei‐An Lai, et al. (2006). Encapsulation of plant growth‐promoting bacteria in alginate beads enriched with humic acid. Biotechnology and Bioengineering, 95(1), 76-83. https://doi.org/10.1002/bit.20957

Encapsulation of plant growth‐promoting bacteria in alginate beads enriched with humic acid

You May Also Like